We narrowed to 159,868 results for: addgene

1. pSNAPf-Nup43

   Plasmid

   #98276

   Purpose

   mammalian expression of SNAPf-Nup43

   Depositor

   Mike Heilemann

   Article

   Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

   Insert

   Nup43 (NUP43 Human)
   

   Use

   Tags

   SNAPf-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHPyV7-713a

   Plasmid

   #24728

   Depositor

   Christopher Buck

   Article

   Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

   Insert

   Full genome of Human polyomavirus 7
   

   Use

   Viral clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. ZAK (ZAKA-c057)

   Plasmid

   #98225

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ZAKA (MAP3K20 Human)
   

   Use

   Baculovirus

   Tags

   His-6-TEV

   Expression

   Mutation

   pfam00069:Pkinase 16-257

   Promoter

   polyhedrin

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNup133-Ypet

   Plasmid

   #98283

   Purpose

   mammalian expression of Nup133-Ypet

   Depositor

   Mike Heilemann

   Article

   Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

   Insert

   Nup133 (NUP133 Human)
   

   Use

   Tags

   YPet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUG+p97W (C2)

   Plasmid

   #74018

   Purpose

   lentiviral expression of EGFP-SAP97beta fusion

   Depositor

   Robert Malenka, Oliver Schluter, Weifeng Xu

   Article

   Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

   Insert

   SAP97 beta isoform
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIgnaviCas9

   Plasmid

   #127595

   Purpose

   Expresses IgnaviCas9 in E. coli

   Depositor

   Stephen Quake

   Article

   Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

   Insert

   IgnaviCas9
   

   Use

   Tags

   6xHis Tag and Maltose binding protein

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHPyV6-607a

   Plasmid

   #24727

   Depositor

   Christopher Buck

   Article

   Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

   Insert

   Full genome of Human polyomavirus 6
   

   Use

   Viral clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBabe NEO mRFP1

   Plasmid

   #98397

   Purpose

   Retroviral vector for constitutive mRFP1 expression

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   mRFP1
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEVY-T

   Plasmid

   #51232

   Purpose

   bi-directional expression vector for yeast, constitutively active, TRP1 selection

   Depositor

   Charles Miller

   Article

   Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD/ADH1

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-dCas9-LSD1-K661A-Hygro

    Plasmid

    #104414

    Purpose

    transient expression of dCas9-dLSD1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dLSD1 (KDM1A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    IRATp970A0364D (Source Bioscience) has a P405H ch…

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDB4280

    Plasmid

    #98699

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. ATG5 sgRNA

    Plasmid

    #207555

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ATG5 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    AACTTGTTTCACGCTATATC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBEVY-L

    Plasmid

    #51226

    Purpose

    bi-directional expression vector for yeast, constitutively active, LEU2 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD/ADH1

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDB4281

    Plasmid

    #98700

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLJM1-PCYT2

    Plasmid

    #81074

    Purpose

    Expresses PCYT2 in mammalian cells

    Depositor

    Erin O'Shea

    Article

    Chidley et al Elife. 2016 Jul 12;5. pii: e14601. doi:

    Insert

    phosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. ULK1 KO sgRNA

    Plasmid

    #207561

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CCCGCCTGCGCCATGGAGCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe NEO Venus

    Plasmid

    #98396

    Purpose

    Retroviral vector for constitutive Venus expression

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    Venus
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Venus-Bik-DD-pEGFP-C1

    Plasmid

    #177429

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Threonine 33 and Serine 35 replaced with aspartic…

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPR_eGFP_497

    Plasmid

    #111824

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 477-497
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. HTATIP (2OU2)

    Plasmid

    #33338

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HTATIP (KAT5 Human)
    

    Use

    Tags

    Hexahistidine tag with Thrombin cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 11, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits