Addgene

We narrowed to 8,156 results for: gnal

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TagBFP2-PIP4K2AD273K

Plasmid

#202727

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:PIP4K2A(D266K) (PIP4K2A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Lyn11C32S-PIP4K2A

Plasmid

#202748

Purpose

weakly plasma membrane targeted fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:LYN (1-11) (C3S):PIP4K2A (PIP4K2A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

Plasmid

#121944

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

U6 promoters

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2

Plasmid

#227775

Purpose

Expression of beta actin driven BFP with multiple cloning site at 3' end. Also contains heat-shock activated mCherry reporter and CreERT2.

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

BFP-MCS
mCherry-t2A-CreERT2

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Olactb and hsp70l

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEFmEGFP-mDia2

Plasmid

#25407

Depositor

Arthur Alberts

Article

Tominaga et al Mol Cell. 2000 Jan . 5(1):13-25.

Insert

mDia2 (Diaph3 Mouse)

Use

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

Encodes mDia2

Promoter

Available since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only

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Halo-MTS

Plasmid

#124315

Purpose

Expresses mitochondria specific HaloTag protein

Depositor

Jin Wang

Article

Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

Insert

Halo tag

Use

Tags

Mito specific seq

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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HA-GLUT5 in pRK5

Plasmid

#154205

Purpose

N-terminal HA-tagged GLUT5 cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

SLC2A5 (SLC2A5 Human)

Use

Tags

N terminal HA tag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBW101ParsR-gfp

Plasmid

#78636

Purpose

pSB3K3 carrying J101-rbs32-arsR-t-ParsR-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J101-rbs32-arsR-t-ParsR-30gfp-t

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
TrkB mEGFP

Plasmid

#83952

Purpose

FRET donor for dynamically measuring TrkB activity in neurons

Depositor

Ryohei Yasuda

Article

Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

Insert

Tropomyosin related kinase B (Ntrk2 Mouse)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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hE-cadherin-pcDNA3

Plasmid

#45769

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin (CDH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

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CMVTO-p450-FRB-cTEVP

Plasmid

#179676

Purpose

ER-associated FRB split-cTEVP (Rapalog inducible)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

FRB (MTOR Human, Synthetic)

Use

Tags

cTEVP

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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GCaMP-AMPK-SPARK

Plasmid

#226257

Purpose

Dual reporter of Ca2+ levels and AMPK activity

Depositor

Roland Malli

Article

Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

Insert

GCaMP-AMPK-SPARK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-EF1a-mCherry-MinD-IRES-Puro

Plasmid

#213112

Purpose

Expression of mCherry-MinD in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

mCherry-MinD (minD Escherichia coli)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p160Tol2-her4.3:switchCas9

Plasmid

#227680

Purpose

Expression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

loxP-dsRed-loxP-Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6 and her4.3

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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HA-hULK1

Plasmid

#31963

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (ULK1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only

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pNTI821

Plasmid

#216630

Purpose

Tet-inducible Cal-ID (V5) for Sleeping Beauty transposon system

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID

Use

Sleeping beauty transposon system

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI934

Plasmid

#216637

Purpose

Cal-ID-PM (V5) Plasma membrane-localized Cal-ID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID-PM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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NbV5-HALOTAG

Plasmid

#201482

Purpose

Indirect detection of V5-tagged protein using the HaloTag protein labeling technology.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-HALOTAG

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagB 54L

Plasmid

#19314

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

T54L

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pNTI825

Plasmid

#216633

Purpose

Cal-ID (V5) for AAV production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID

Use

AAV

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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