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We narrowed to 16,241 results for: grna

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Showing: 2181 - 2200 of 16241 results

  6. PB-U6-gRNA-Ef1a-CRISPRi-blast

    Plasmid
    #262893
    Purpose
    piggyBac vector to transduce both U6-driven gRNA and Ef1a-driven CRISPRi with blasticidin resistance
    Depositor
    Ronald Hart
    Inserts
    U6-gRNA Cloning Site (BsmB1 GoldenGate)
    KRAB-dCas9-MeCP2
    Use
    CRISPR; Piggybac
    Tags
    blasticidin S deaminase
    Expression
    Mammalian
    Promoter
    Ef1a and U6
    Available since
    Oct. 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    262893-plasmid-map-sequence-id-524313

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  7. pMOD_B2121

    Plasmid
    #91065
    Purpose
    Module B, Promoter: GmUbi, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Promoter
    GmUbi
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91065-plasmid-map-sequence-id-181862

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  9. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196076
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196076-plasmid-map-sequence-id-388979

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  10. pRP_8xgRNA-FLuc

    Plasmid
    #135937
    Purpose
    Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.
    Depositor
    Antonio Amelio
    Publication
    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    minCMV
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    135937-plasmid-map-sequence-id-271493

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  11. pDIRECT_25G

    Plasmid
    #91144
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91144-plasmid-map-sequence-id-182272

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Showing: 2181 - 2200 of 16241 results