We narrowed to 4,299 results for: pme

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hRad51(K133R)

Plasmid

#125571

Purpose

Expresses the hRad51(K133R) protein in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51 (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Mutation

K133R

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd8

Plasmid

#42260

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 8 (Fzd8 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green

Plasmid

#170048

Purpose

Tri-Responsive Reporter Gene Construct for redgreen, triple red, and double green

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mScarlet
COmScarlet
mRuby3
mWasabi dN
mWasabi CO

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMpGWB101

Plasmid

#68555

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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Mouse ZP3 promoter (JD#445)

Plasmid

#14634

Depositor

Jurrien Dean

Article

Rankin et al Development. 1998 Jul . 125(13):2415-24.

Insert

ZP3 promoter (Zp3 Mouse)

Expression

Bacterial

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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Spacer Switch Plasmid

Plasmid

#215346

Purpose

Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

Depositor

Hank Seifert

Article

Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

Insert

OpaD spacer; Chloramphenicol Resistance Cassette

Use

CRISPR

Expression

Bacterial

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-mBcl11b-MAR4

Plasmid

#178188

Purpose

Lucifease reporter driven by MAR4 regulatory sequences of mouse Bcl11b

Depositor

Katsuhiko Ono

Article

Nomura et al Cell Rep. 2018 Mar 20;22(12):3142-3151.

Insert

Bcl11b MAR4

Use

Luciferase

Expression

Mammalian

Promoter

SV40 promoter

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

Plasmid

#227774

Purpose

Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

loxP-dsR-loxP

Use

CRISPR

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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FsC_pET29b

Plasmid

#215405

Purpose

Expression construct for FsC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

FsC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd3

Plasmid

#42255

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 3 (Fzd3 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
CBR1_pCSdest

Plasmid

#53826

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CBR1 (CBR1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pJ204-loxP-100RPU.2-coEPO-lox2272

Plasmid

#154828

Purpose

Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietin

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

coEPO

Use

Cre/Lox

Expression

Mammalian

Mutation

Codon-optimized (Cricetulus griseus)

Promoter

100RPU.2

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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ThcCut1_pET29b

Plasmid

#215429

Purpose

Expression construct for ThcCut1 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

ThcCut1

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2

Plasmid

#227775

Purpose

Expression of beta actin driven BFP with multiple cloning site at 3' end. Also contains heat-shock activated mCherry reporter and CreERT2.

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

BFP-MCS
mCherry-t2A-CreERT2

Use

Zebrafish expression

Promoter

Olactb and hsp70l

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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UMG-SP-3_pET29b

Plasmid

#215435

Purpose

Expression construct for UMG-SP-3 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-3

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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NfPolyA_pET21b

Plasmid

#215413

Purpose

Expression construct for NfPolyA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NfPolyA

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPM1003

Plasmid

#167550

Purpose

Encodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Insert

Vibrio cholerae O antigens genes of the Ogawa serotype

Use

Cosmid

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHA-mLRH-1

Plasmid

#16342

Depositor

Austin Cooney

Article

Gu et al Mol Cell Biol. 2005 May . 25(9):3492-505

Insert

LRH-1 (Nr5a2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only
GFP-nArgBP2

Plasmid

#74514

Purpose

Express GFP-nArgBP2 fusion protein in mammalian cells

Depositor

Guoping Feng

Article

Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

Insert

nArgBP2 (Sorbs2 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-ATP

Plasmid

#225247

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-ATP

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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