We narrowed to 13,223 results for: Ada
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Plasmid#113490PurposeExpression of human PNGase with C-terminal strep-HA tagDepositorInsertPNGase (NGLY1 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable SinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub(TA)-YPet-NES
Plasmid#84636PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub(TA)-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 myc card9 mutant
Plasmid#16254DepositorInsertcard9 mutant (CARD9 Human)
UseTagsmycExpressionMammalianMutationS528A, T531A, T533A, S536APromoterAvailable SinceNov. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-iCoChR++
Plasmid#98173Purposeartificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.DepositorInsertSynthetic construct iCoChR++ gene
UseTagsmCherryExpressionMammalianMutationA59S, E83N, E90Q, E101S, Q117R, E123S, V242R, T24…PromoterCMV (+enhancer)Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.SFFV.dTomato-miR30n
Plasmid#90334PurposemiR30 based shMir knockdownDepositorInsertsdTomato
na
SFFV
miR30n
UseLentiviralTagsExpressionMutationPromoterAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL2 p15 (-756/+68) SBR2 Mut 3' box
Plasmid#15711DepositorInsertp15 promoter (CDKN2B Human)
UseLuciferaseTagsExpressionMammalianMutationMut 3' box in SBR2. The 3' box contains…PromoterAvailable SinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
UbG76V-eGFP-V5His_15aa
Plasmid#23969DepositorInsertUb-G76V-eGFP-V5HIS_15aa (UBB Human)
UseTagseGFPExpressionMammalianMutationG76V mutation in Ubiquitin. Ub fused to N-termin…PromoterAvailable SinceApril 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T236A/T240A
Plasmid#111458PurposeTransient expression of Flag-tagged human Sox9 with T236A/T240A mutationDepositorAvailable SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-PLAA-Strep-HA
Plasmid#113484PurposeExpression of human PLAA with C-terminal strep-HA tagDepositorInsertPLAA (PLAA Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
(p)odr-3::GFP::EGL-4
Plasmid#100897PurposeGFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.DepositorInsert56-2741: odr-3 promoter
UseTagsExpressionWormMutationPromoterodr-3Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKK-HA-TEV
Plasmid#105769PurposeExpression of your protein of interest in fusion with HA at the N-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsHA-TEVExpressionMammalianMutationPromoterAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-HA
Plasmid#105785PurposeExpression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-HAExpressionMammalianMutationPromoterAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-APPL1-ΔPTB
Plasmid#59768PurposeExpresses APPL1 with PTB domain deletedDepositorInsertAPPL1 (APPL1 Human)
UseTagsEGFPExpressionMammalianMutationdeleted amino acids 467-709PromoterCMVAvailable SinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
XE257 pCDNA3.1(zeo)-hDsh3-truncDEP
Plasmid#16752DepositorInsertFLAG-hDsh3-trunc before DEP (DVL3 Human)
UseTagsflagExpressionMammalianMutationtruncated before DEPPromoterAvailable SinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRetroSuper shAngptl4 hygro
Plasmid#19154DepositorInsertsmall hairpin RNA against angiopoietin-like 4 (ANGPTL4 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pONSY-CoNMM:mCherry
Plasmid#111878PurposeThis plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.DepositorInsertCapsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
UseCapsaspora owczarzakiTagsmCherryExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Elafin
Plasmid#18102DepositorAvailable SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1Gh
Plasmid#44507DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Luc-Sox2-3'UTR
Plasmid#63895PurposeLuciferase reporter of mouse wildtype Sox2 3'UTRDepositorInsertSRY (sex determining region Y)-box 2 (Sox2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable SinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-iC++
Plasmid#98165Purposeartificial anion conducting channelrhodopsin (aACR). Activation max 488 nm; off-kinetics 35 ms. Codon optimized for mammalian expression.DepositorInsertSynthetic construct iC++ gene
UseTagsmCherryExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, V242R, T24…PromoterCMV (+enhancer)Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only