Addgene

We narrowed to 10,507 results for: CHL

Showing: 2201 - 2220 of 10507 results

Results per page:

Sort results by:

pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b

Plasmid

#162816

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_9b

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_9b(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

Plasmid

#162817

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF6

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF6(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

Plasmid

#162818

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF7b

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF7b(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

Plasmid

#162819

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF10

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF10(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14

Plasmid

#162820

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF9c/14

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF9c/14(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt

Plasmid

#162821

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF3b_alt

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF3b_alt(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)

Plasmid

#162822

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF3b(CoV1h)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF3b(CoV1h)(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

Plasmid

#162805

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA_IRES_Blasticidin

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet1-tglCDEF

Plasmid

#134677

Purpose

Expresses TglC, TglD, TglE, TglF in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Inserts

TglC
TglD
TglE
TglF

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet1-ammA(∆Trp56)

Plasmid

#134683

Purpose

Expresses His6-AmmA(∆Trp56) in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammA(delW56)

Use

Tags

His6

Expression

Bacterial

Mutation

deletion of W56

Promoter

T7

Available since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCKan-yibDp-SBS-mCherry

Plasmid

#134598

Purpose

Low Phosphate inducible SBS (bonryl-diphosphate) synthase with C-terminal mCherry)

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

SBS-mCherry

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR207-OEP7(1–50)

Plasmid

#100601

Purpose

Gateway entry vector for AtOEP7(1–50)

Depositor

Yutaka Kodama

Article

Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

Insert

OEP7(1–50) (OEP7 Mustard Weed)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pOVC1_X_CrPH-LOV

Plasmid

#131186

Purpose

Expresses CrPh-LOV domain (Chlamydomonas reinhardtii) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

CrPH-LOV

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOVC1_CrPH-LOV_X

Plasmid

#131175

Purpose

Expresses CrPh-LOV domain (Chlamydomonas reinhardtii) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

CrPH-LOV

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
JK104: pMVP-DEST

Plasmid

#121851

Purpose

pMVP destination vector, empty expression plasmid backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mCm7RNA6A

Plasmid

#84367

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 m7 mutant knockin

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2 m7-gRNA6

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
ARK1a

Plasmid

#81016

Purpose

E.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Inserts

MevTo-BBa1002-pTrc-Mkco-PMKco
PMD-NudB

Use

Tags

Expression

Mutation

Promoter

LacUV5 and Trc promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYB3-human PUM1-HD MUT6-3 (N1043S-Y1044N-Q1047E)-intein-CBD

Plasmid

#73298

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Wang et al Cell. 2002 Aug 23;110(4):501-12.

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Use

Tags

intein

Expression

Bacterial

Mutation

N1043S-Y1044N-Q1047E

Promoter

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pET151-hUCH37 (ISF2)

Plasmid

#61930

Purpose

Expression of hUCH37 (ISF2) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hUCH37 (ISF1) (UCHL5 Human)

Use

Tags

6x HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pY010 (pcDNA3.1-hAsCpf1)

Plasmid

#69982

Purpose

Expresses humanized AsCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hAsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge