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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 28,061 results for: GFP

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Showing: 2201 - 2220 of 28061 results

  2. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

    Plasmid
    #99248
    Purpose
    single-vector system with EGFP reporter for use in CROPseq knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99248-plasmid-map-sequence-id-191832

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  4. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid
    #113865
    Purpose
    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
    Depositor
    Manfred Ogris
    Publication
    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d
    Inserts
    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113865-plasmid-map-sequence-id-220242

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  5. lentiCRISPR - EGFP sgRNA 3

    Plasmid
    #51762
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 3
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51762-plasmid-map-sequence-id-79380

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  10. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid
    #185912
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8e-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    185912-plasmid-map-sequence-id-367362

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  12. pLenti-TRE-GFP-Blast

    Plasmid
    #135673
    Purpose
    Dox-inducible expression (TRE promoter) of GFP cDNA (control for other cDNAs), Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    GFP
    Use
    Lentiviral
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135673-plasmid-map-sequence-id-327577

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  16. pCIP-GFP

    Plasmid
    #79012
    Purpose
    For mammalian expression of GFP.
    Depositor
    Reuben Shaw
    Publication
    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP
    Expression
    Mammalian
    Promoter
    pCAGGs
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pMRX-No-HaloTag7-mGFP-LC3-mRFP

    Plasmid
    #184902
    Purpose
    Stable expression of HaloTag7-mEGFP-LC3-mRFP in mammalian cells to measure autophagic flux
    Depositor
    Noboru Mizushima
    Publication
    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LC3B
    Tags
    HaloTag7, monomeric EGFP, and monomeric RFP
    Expression
    Mammalian
    Available since
    June 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184902-plasmid-map-sequence-id-364036

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  19. pDAS12489_PEAR-GFP_2in1_2.0

    Plasmid
    #177186
    Purpose
    PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid
    Depositor
    Ervin Welker
    Publication
    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    EGFP split with an intron between amino acids 95-96
    pegRNA targeting its own plasmid
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    disrupted 5' splice site
    Promoter
    CMV and U6
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    177186-plasmid-map-sequence-id-348478

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  20. pSBBi-Pur GFP-TM

    Plasmid
    #139185
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.
    Depositor
    Neal Devaraj
    Insert
    GFP-TM
    Use
    Transposon
    Expression
    Mammalian
    Mutation
    fused to membrane anchor
    Promoter
    EF1α
    Available since
    March 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    139185-plasmid-map-sequence-id-309371

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Showing: 2201 - 2220 of 28061 results