We narrowed to 3,906 results for: SPL

1. pU6_(GLuc)_INT[3xPP7_SL]

   Plasmid

   #68424

   Purpose

   Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct bearing three PP7 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. YFP-Cdc42(N17)

   Plasmid

   #11400

   Depositor

   Joel Swanson

   Article

   Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

   Insert

   cell division cycle 42 (CDC42 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Obtained with Threonine 17 changed to Asparagine.

   Promoter

   Available since

   Feb. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Myc-His RTN3A1

   Plasmid

   #31087

   Depositor

   Jimtong Horng

   Article

   Tang et al J Biol Chem. 2007 Feb 23. 282(8):5888-98

   Insert

   reticulon 3 (RTN3 Human)
   

   Use

   Tags

   Myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMM2-hPMCA2w/b

   Plasmid

   #47583

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   PMCA2w/b (ATP2B2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMM2-hPMCA2z/b

   Plasmid

   #47581

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   PMCA2z/b (ATP2B2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-21a_2xNLS_LbCpf1_protein_expression

   Plasmid

   #114366

   Purpose

   Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

   Insert

   2xNLS_LbCpf1
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG SMG1 T3550A/S3556A

   Plasmid

   #199574

   Purpose

   Expresses FLAG-tagged mutated SMG1

   Depositor

   Michael Kastan

   Article

   McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

   Insert

   SMG1 (SMG1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T3550A and S3556A

   Promoter

   CMV

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

   Plasmid

   #172065

   Purpose

   Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   mTagBFP2(11x7)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA3-Nrxn1beta AS4(+)-Fc

   Plasmid

   #59314

   Purpose

   Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

   Depositor

   Peter Scheiffele, Tito Serafini

   Article

   Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

   Insert

   Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
   

   Use

   Tags

   Fc (from human IgG)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. TRE-UBC-mCD59-T2A-GFP

    Plasmid

    #205451

    Purpose

    lentiviral plasmid for expression of mouse CD59

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    mCD59 (Cd59a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. (424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A)

    Plasmid

    #168191

    Purpose

    HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutation

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nLag6-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGLOW79

    Plasmid

    #177339

    Purpose

    C. elegans mNeonGreen co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-3::mNeonGreen
    

    Use

    Tags

    mNeonGreen

    Expression

    Worm

    Mutation

    Promoter

    myo-3

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. YFP-Rac1(N17)

    Plasmid

    #11395

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Threonine 17 mutated to Asparagine.

    Promoter

    Available since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. DT40

    Plasmid

    #80415

    Purpose

    Expresses 40 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 40 interrupted CTG repeats (DMPK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRCHisB-TRF2∆M

    Plasmid

    #50489

    Purpose

    E. Coli N-term HexHis Tagged Protein Expression Vector

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TRF2 Delta Myb (TERF2 Human)
    

    Use

    Tags

    6x His, T7 tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Contains amino acids 3-437

    Promoter

    pTrc

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. anxA9-GFP

    Plasmid

    #107198

    Purpose

    Expresses human annexin A9-GFP fusion protein for live cell imaging

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Goebeler et al FEBS Lett. 2003 Jul 10;546(2-3):359-64.

    Insert

    annexin A9 (ANXA9 Human)
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA flag-Pcbp2

    Plasmid

    #184028

    Purpose

    Codon optimized Pcbp2 clone with N-terminal flag tag

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Pcbp2 (Pcbp2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    CMV

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393

    Plasmid

    #216380

    Purpose

    Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)

    Depositor

    William Weis

    Article

    Bruguera et al bioRxiv 2024.02.03.578714

    Insert

    Frizzled-4 (Fzd4 Mouse)
    

    Use

    Baculovirus

    Tags

    3C-splitGFP1to10opt-ETVV and HA signal sequence-F…

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. YFP-Rac1(L61)

    Plasmid

    #11401

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Glutamine 61 mutated to Leucine.

    Promoter

    Available since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pENTR_hEMSY

    Plasmid

    #125156

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    EMSY (EMSY Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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