We narrowed to 15,064 results for: pET

1. pEE006

   Plasmid

   #176763

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   102
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEE007

   Plasmid

   #176764

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   103
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEE008

   Plasmid

   #176765

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   104
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEE009

   Plasmid

   #176766

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   201
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEE010

   Plasmid

   #176767

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   202
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGFP-HsSyx3

   Plasmid

   #179289

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + GFP + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Use

   Tags

   His6 + GFP + TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   residues 393-792 from accession number NP_0010361…

   Promoter

   T7

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHsSyx3-MBP

   Plasmid

   #179286

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Use

   Tags

   TEV protease cleavage site + mature maltose bindi…

   Expression

   Bacterial

   Mutation

   residues 393-792 from accession number NP_0010361…

   Promoter

   T7

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. M3K5_HUMAN_D0

   Plasmid

   #79710

   Purpose

   This plasmid encodes the kinase domain of M3K5. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, John Chodera, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   M3K5 (MAP3K5 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. E2AK2_HUMAN_D0

   Plasmid

   #79712

   Purpose

   This plasmid encodes the kinase domain of E2AK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, John Chodera, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   E2AK2 (EIF2AK2 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. CSKP_HUMAN_D0

    Plasmid

    #79714

    Purpose

    This plasmid encodes the kinase domain of CSKP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CSKP (CASK Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. DMPK_HUMAN_D0

    Plasmid

    #79717

    Purpose

    This plasmid encodes the kinase domain of DMPK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    DMPK (DMPK Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. KC1G1_HUMAN_D0

    Plasmid

    #79691

    Purpose

    This plasmid encodes the kinase domain of KC1G1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KC1G1 (CSNK1G1 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. CSK22_HUMAN_D0

    Plasmid

    #79704

    Purpose

    This plasmid encodes the kinase domain of CSK22. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CSK22 (CSNK2A2 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. FL

    Plasmid

    #120522

    Purpose

    Firefly luciferase reporter

    Depositor

    Petr Svoboda

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. U6-MosIR

    Plasmid

    #120529

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Mos (Mos Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. H1-Mos-6U

    Plasmid

    #120531

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Mos (Mos Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    A>G (see comments)

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CMV-MosIR

    Plasmid

    #120523

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Mos (Mos Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. biGBac Kit

    Plasmid Kit

    #1000000088

    Purpose

    Generation of baculoviral transfer vectors coding up to 25 protein complex subunits in two Gibson assembly steps. Multisubunit protein complexes can be generated in baculovirus-infected insect cells.

    Depositor

    Jan-Michael Peters

    Available Since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pSV40_HA-mTarbp2

    Plasmid

    #120539

    Purpose

    protein expression

    Depositor

    Petr Svoboda

    Insert

    TARBP2 (Tarbp2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. U6-Mos-as

    Plasmid

    #120534

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Mos (Mos Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    A>G (see depositor comments)

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. U6-Mos-s

    Plasmid

    #120533

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Mos (Mos Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    A>G (see depositor comments)

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CAG-EGFP-MosMos

    Plasmid

    #120515

    Purpose

    ssRNA expression

    Depositor

    Petr Svoboda

    Insert

    Mos (Mos Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CMV-Mos-as

    Plasmid

    #120526

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Mos (Mos Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CMV-Mos-s

    Plasmid

    #120525

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Mos (Mos Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    A>G (see comments)

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSV40_HA-mPACT

    Plasmid

    #120537

    Purpose

    protein expression

    Depositor

    Petr Svoboda

    Insert

    PACT (Prkra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RL-Mos

    Plasmid

    #120519

    Purpose

    Renilla luciferase reporter

    Depositor

    Petr Svoboda

    Insert

    Renilla luciferase (Mos Synthetic, Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDESTsvaw

    Plasmid

    #32318

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    ccdB
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDESThaw

    Plasmid

    #32317

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    ccdB
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CAG-EGFP-MosIR

    Plasmid

    #120516

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Mos (Mos Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    L170P in second MosIR repeat (see comments below)

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pENTR L3-4XmCherry-HA-L2

    Plasmid

    #32315

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    4XmCherry-HA
    

    Use

    Gateway multisite entry vector

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pENTR L3-4XeGFP-Myc-L2

    Plasmid

    #32316

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    4XeGFP-Myc
    

    Use

    Gateway multisite entry vector

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pENTR R4-mCherry-R3

    Plasmid

    #32311

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    mCherry
    

    Use

    Gateway multisite entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pENTR R4-eGFP-R3

    Plasmid

    #32312

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    eGFP
    

    Use

    Gateway multisite entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pENTR L3-mCherry-HA-L2

    Plasmid

    #32313

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    mCherry-HA
    

    Use

    Gateway multisite entry vector

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. U6-Lin28aIR

    Plasmid

    #120530

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pENTR L5-QF-L2

    Plasmid

    #32305

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    QF
    

    Use

    Gateway multisite entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. H1-Lin28-6U

    Plasmid

    #120532

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. EH1

    Bacterial Strain

    #102804

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC ilvA genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

    Bacterial Resistance

    None

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

39. pcDNA3.1-mArg1

    Plasmid

    #34573

    Depositor

    Peter Murray

    Insert

    Arg1 (Arg1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMIY-Arg1

    Plasmid

    #34580

    Depositor

    Peter Murray

    Insert

    Arg1 (Arg1 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

41. CAG-EGFP-Elavl2IR

    Plasmid

    #120518

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Elavl2 (Elavl2 Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pENTR L1-20XUAS-R5

    Plasmid

    #32302

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    20XUAS
    

    Use

    Gateway multisite entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pENTR L1-20XUAS-L4

    Plasmid

    #32307

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    20XUAS
    

    Use

    Gateway multisite entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pMIY-Arg1-Flag

    Plasmid

    #34581

    Depositor

    Peter Murray

    Insert

    Arg1 (Arg1 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

45. pENTR L3-eGFP-Myc-L2

    Plasmid

    #32314

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    eGFP-Myc
    

    Use

    Gateway multisite entry vector

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pENTR L1-5XQUAS-R5

    Plasmid

    #32303

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    5XQUAS
    

    Use

    Gateway multisite entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pENTR L1-5XQUAS-L4

    Plasmid

    #32308

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    5XQUAS
    

    Use

    Gateway multisite entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pENTR R4-QF-R3

    Plasmid

    #32310

    Depositor

    Steve Stowers

    Article

    Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

    Insert

    QF
    

    Use

    Gateway multisite entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. CMV-Lin28a-as

    Plasmid

    #120528

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. CAG-EGFP-Lin28IR

    Plasmid

    #120517

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge