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SBP-GFP-Tac

Plasmid

#162505

Purpose

Expresses GFP tagged Tac in a RUSH vector in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

Tac (IL2RA Human)

Tags

GFP and SBP

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pTS2355_Tier3(PB)-iRFP_PuroR

Plasmid

#169654

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven iRFP670 and PuroR expression

Expression

Mammalian

Promoter

PRPBSA

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro LAP2

Plasmid

#15740

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LAP2 (CEBPB Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
Gal4-PGC1 alpha

Plasmid

#8892

Depositor

Bruce Spiegelman

Article

Puigserver et al Cell 1998 Mar 20;92(6):829-39.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Tags

Gal4 DBD

Expression

Mammalian

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only
pCDNA3-Myc3-Cul3 delta N41

Plasmid

#21590

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

cullin 3 (CUL3 Human)

Tags

myc3

Expression

Mammalian

Mutation

Residues 34-74 deleted from N terminus.

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only
Myc-E-Syt3

Plasmid

#66835

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt3 (ESYT3 Human)

Tags

Myc

Expression

Mammalian

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3/mArf1(T31N)-HA

Plasmid

#79411

Purpose

Expresses C-terminally HA-tagged Arf1(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf1 (Arf1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 31 to Asparagine

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1 hBRM

Plasmid

#1956

Depositor

Robert Kingston

Article

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BRM (SMARCA2 Human)

Tags

flag

Expression

Insect

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
Arf Q71L CFP

Plasmid

#128149

Purpose

Expression of Arf Q71L

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 2002 May 9;417(6885):187-93. doi

Insert

Arf1 (ARF1 Human)

Expression

Mammalian

Mutation

Q71L

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pEBB HA cIAP1 H588A

Plasmid

#38233

Depositor

Colin Duckett

Article

Csomos et al Biochem J. 2009 Apr 28;420(1):83-91.

Insert

cIAP1 H588A (BIRC2 Human)

Tags

HA

Expression

Mammalian

Mutation

H588A

Promoter

EF1a

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pmyc-GFP-TNRC6A-(1-1223)

Plasmid

#42013

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa1-1223

Promoter

CMV

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13B

Plasmid

#134626

Purpose

Mammalian expression of mouse KIF13B 442-1843

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF13B (Kif13b Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL

Plasmid

#169533

Purpose

Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven ER-localized monomeric red fluorescent protein mRuby2

Expression

Mammalian

Promoter

PhCMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC3

Plasmid

#22976

Depositor

Marc Montminy

Article

Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

Insert

TORC3 (CRTC3 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pMSCV-human-pro-IL-18

Plasmid

#214317

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Promoter

MESV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSG5-ERRbeta2

Plasmid

#52186

Purpose

Expresses human ERRbeta2 splice variant in mammalian cells

Depositor

Rebecca Riggins

Article

Heckler et al Cell Cycle. 2014 Dec 12:0.

Insert

Estrogen-related receptor beta (ESRRB Human)

Expression

Mammalian

Mutation

wild type sequence; codon optimized to exclude Ec…

Promoter

SV40

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA/golgin-160/myc

Plasmid

#180632

Purpose

Expression of WT golgin-160

Depositor

Carolyn Machamer

Article

Hicks et al J Biol Chem. 2002 Sep 27. 277(39):35833-

Insert

golgin-160 (GOLGA3 Human)

Tags

Myc-His

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBS hBAF53a

Plasmid

#17879

Depositor

Jerry Crabtree

Article

Zhao et al Cell. 1998 Nov 25. 95(5):625-36.

Insert

BAF53a (ACTL6A Human)

Expression

Bacterial

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pTS2311-Tier1-spacer-PPGK-NLuc_TB

Plasmid

#169552

Purpose

Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-NanoLuc Luciferase - mTagBFP fusion

Expression

Mammalian

Promoter

PPGK

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAGM51559

Plasmid

#153226

Purpose

Control plasmid for knockout of TRY and CPC genes

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Cas9, guide RNA for TRY and CPC, FAST marker

Use

Synthetic Biology

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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