We narrowed to 11,353 results for: nls

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pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

Plasmid

#115139

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3.1 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn_NLS-His-rsCaMPARI-mRuby3

Plasmid

#122092

Purpose

AAV plasmid for expressing rsCaMPARI in neurons, nucleus localized

Depositor

Eric Schreiter

Article

Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

Insert

rsCaMPARI

Use

AAV

Tags

NLS-His and mRuby3

Promoter

hsyn (synapsin-1)

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)

Plasmid

#101105

Purpose

Encodes dCas9(C) (without VP64) fused to VEGFR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

Plasmid

#127365

Purpose

Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Tags

GS linker, NLS, V5, and YFP

Expression

Plant

Mutation

R118G

Promoter

Arabidopsis UBQ10 promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSLIK 3XFLAG-MKK4-EE-NLS hygro

Plasmid

#87779

Purpose

Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only
pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

Plasmid

#81206

Purpose

CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

pGinB-8X(GGS)-dCas9-FLAG-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pTS1026-Tier1-PhCMV-3xNLS-iRFP670

Plasmid

#169532

Purpose

Tier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nucleus-localized far-red fluorescent protein iRFP670

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-tdiRFP670-2A-LBR

Plasmid

#82620

Purpose

Expresses NLS-tdiRFP-2A-LBR in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

NLS-tdiRFP670-LBR (Lbr Mouse, Synthetic)

Use

Piggybac vector

Expression

Mammalian

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Construct 7 - CMVp-Cas9-3xNLS-HSVpA

Plasmid

#81247

Purpose

Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expression

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

Plasmid

#115140

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

Plasmid

#115138

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA2 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pZLCv2-gD4Z4-1-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106352

Purpose

CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

Depositor

Stephen Tapscott

Article

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

D4Z4 gRNA-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGGEV_4'_+nls-mCherry-Flag-_+1_BK+

Plasmid

#49313

Purpose

Golden Gate entry vector: Position 4', Flag tagged nls-mCherry with start and stop codon

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Insert

nls-mCherry-Flag

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NLS-IRES-hrGFP

Plasmid

#107543

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NLS (APP Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13-IDR-YFP-NLS

Plasmid

#145277

Purpose

Mammalian Expression of Nuclear HOXD13 IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13-IDR (HOXD13 Human)

Expression

Mammalian

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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3xNLS-NLP-cMyc-cMyc enAspCas12a

Plasmid

#182127

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc enAspCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106355

Purpose

CRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus

Depositor

Stephen Tapscott

Article

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

MYOD1 gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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ubc-nls-pcp-5xSunTag (SRv1)

Plasmid

#185797

Purpose

pcp-5xSunTag component for SunRISER SRv1

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

pcp-5xSunTag

Tags

FactorXa site, HA, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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Crimson/P2A-NLS/HA-HumBeta (pSLIK2)

Plasmid

#113857

Purpose

For Doxycycline-inducible expression of Humanized Beta synaptase gene, with red fluorescent protein gene E2-Crimson-P2A-Humanized Beta synaptase gene

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

Humanized Beta

Use

Lentiviral

Tags

P2A/Crimson

Mutation

"humanized" Beta gene

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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3xNLS-NLP-cMyc-cMyc AspCas12a

Plasmid

#182122

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc AspCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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