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mCherry-TMCC1

Plasmid

#120932

Purpose

Expresses human TMCC1 in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Insert

Trans membrane and coiled coil domain containing protein 1 (TMCC1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Golgi-DKAR

Plasmid

#49790

Purpose

FRET-based, Golgi-targeted kinase activity reporter for PKD

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2010 Jul 23;285(30):22748-5

Insert

D kinase activity reporter

Tags

CFP, YFP, and eNOS(1-33)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
3xDBE_RE:NLuc

Plasmid

#178318

Purpose

FOXO NLuc luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

FOXO NLuc Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTM-BPL

Plasmid

#173766

Purpose

Fluorescent labeling of the nuclear envelope

Depositor

Shinji Sueda

Article

Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

Insert

PDGF receptor TM domain, Biotin protein ligase

Tags

FLAG and Signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-INPP4BC842A

Plasmid

#116865

Purpose

Inactive recruitable PI(3,4)P2 4-phosphatase

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

INPP4B (INPP4B Human)

Tags

FKBP12 and mCherry

Expression

Mammalian

Mutation

catalytic cysteine 842 changed to alanine

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-ScVrg4

Plasmid

#102334

Purpose

Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

Insert

YGL225W (VRG4 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCS-H2B-citrine

Plasmid

#53752

Purpose

Contains fusion of human Histone H2B to citrine.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

citrine

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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p5xFbx15 (-544/-527)-TK-Luc/Sox-m1

Plasmid

#15687

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

5 x mutated Fbx15 enhancer (Oct/Sox binding site)

Use

Luciferase

Mutation

Mutated in Sox binding site

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
6xAP-1_RE::Renilla

Plasmid

#178317

Purpose

AP-1 Renilla luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

AP-1 Renilla Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBCCP-GFP-NLS

Plasmid

#173923

Purpose

Fluorescent labeling of the nuclear envelope

Depositor

Shinji Sueda

Article

Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

Insert

Biotin carboxyl carrier protein, AcGFP1

Tags

3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-Cre

Plasmid

#167939

Purpose

human CD81-TEV cleavage site-Cre

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD81 (CD81 Human)

Tags

Cre recombinase and TEV protease cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:GrRenilla

Plasmid

#118067

Purpose

Transcriptional unit for constitutive expression of GrRenilla in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:GreenRenilla:bGHF

Use

Luciferase and Synthetic Biology; Assembly of gb2…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRedF

Plasmid

#118057

Purpose

GBPart - RedF Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Red Firefly Luciferase CDS

Use

Synthetic Biology; Domestication of dna parts for…

Mutation

Red mutant S286Y

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pT2K-p53DN-T2A-copGFP

Plasmid

#109229

Purpose

For Tol2 transposon-mediated stable expression of dominant negative p53 and copGFP

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

dominant negative p53 (Trp53 Mouse)

Tags

copGFP

Expression

Mammalian

Mutation

dominant negative p53

Promoter

CAGGS

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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hSERT-N1-pECFP

Plasmid

#70106

Purpose

mammalian expression of hSERT with C-terminal CFP fusion

Depositor

Harald Sitte

Article

Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

Insert

SERT (SLC6A4 Human)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pCDH-F-PA1

Plasmid

#40618

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

PA1 (PAGR1 Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
pEF/myc/ER FLIPglu-600uDelta13V

Plasmid

#18020

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A in binding protein

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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L1-1147

Plasmid

#13267

Depositor

Vance Lemmon

Article

Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

Insert

L1CAM (L1CAM Human)

Expression

Bacterial

Mutation

Truncated in the juxtamembrane region

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyso-ExRai-AktAR2

Plasmid

#184052

Purpose

Lysosome-targeted ExRai-AktAR2.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Lyso-ExRai-AktAR2

Tags

Lysosome-associated membrane protein 1 (LAMP1).

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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hP2X3-cALFA

Plasmid

#160499

Purpose

For the expression of human P2X3 with ALFA tag at C-terminus in mammalian cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

human P2X3 (P2RX3 Synthetic, Human)

Tags

HRV3C protease cleavage site-ALFA tag-twin Strep …

Expression

Mammalian

Mutation

ΔN5ΔC33-T13P/S15V/V16I

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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