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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 20,002 results for: Gnas

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Showing: 2241 - 2260 of 20002 results

  1. pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc W139A

    Plasmid
    #223560
    Purpose
    Alias: Gαi bONE-GO W139A. Lentiviral vector expressing GINIP-Nluc W139A after a low efficiency IRES downstream of YFP-CAAX placed after the promotor
    Depositor
    Mikel Garcia-Marcos
    Article
    Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457
    Insert
    YFP(Venus) - KRas4b - IRES* - human GINIP W139A - linker(GGGS) - Nluc
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    W139A mutation in GINIP sequence
    Promoter
    CMV
    Available Since
    March 28, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    223560-plasmid-map-sequence-id-443638

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  2. pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc W139A

    Plasmid
    #223560
    Purpose
    Alias: Gαi bONE-GO W139A. Lentiviral vector expressing GINIP-Nluc W139A after a low efficiency IRES downstream of YFP-CAAX placed after the promotor
    Depositor
    Mikel Garcia-Marcos
    Article
    Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457
    Insert
    YFP(Venus) - KRas4b - IRES* - human GINIP W139A - linker(GGGS) - Nluc
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    W139A mutation in GINIP sequence
    Promoter
    CMV
    Available Since
    March 28, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    223560-plasmid-map-sequence-id-443638

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  3. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

    Plasmid
    #233064
    Purpose
    Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.
    Depositor
    Jonathan Javitch
    Article
    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.
    Insert
    ADGRL3 (Adgrl3 Mouse)
    Tags
    ALFA and HiBiT
    Expression
    Mammalian
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233064-plasmid-map-sequence-id-464574

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  4. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

    Plasmid
    #233064
    Purpose
    Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.
    Depositor
    Jonathan Javitch
    Article
    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.
    Insert
    ADGRL3 (Adgrl3 Mouse)
    Tags
    ALFA and HiBiT
    Expression
    Mammalian
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233064-plasmid-map-sequence-id-464574

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  5. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

    Plasmid
    #233063
    Purpose
    Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.
    Depositor
    Jonathan Javitch
    Article
    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.
    Insert
    ADGRL3 (Adgrl3 Mouse)
    Tags
    ALFA and HiBiT
    Expression
    Mammalian
    Mutation
    Threonine 923 to Glycine
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233063-plasmid-map-sequence-id-464572

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  6. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

    Plasmid
    #233063
    Purpose
    Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.
    Depositor
    Jonathan Javitch
    Article
    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.
    Insert
    ADGRL3 (Adgrl3 Mouse)
    Tags
    ALFA and HiBiT
    Expression
    Mammalian
    Mutation
    Threonine 923 to Glycine
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233063-plasmid-map-sequence-id-464572

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  7. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid
    #140982
    Purpose
    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaQ-RLuc8 (GNAQ Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140982-plasmid-map-sequence-id-300744

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  8. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid
    #140982
    Purpose
    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaQ-RLuc8 (GNAQ Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140982-plasmid-map-sequence-id-300744

    Enlarge

  9. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid
    #140973
    Purpose
    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai1-RLuc8 (GNAI1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140973-plasmid-map-sequence-id-300655

    Enlarge

  10. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid
    #140973
    Purpose
    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai1-RLuc8 (GNAI1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140973-plasmid-map-sequence-id-300655

    Enlarge

  11. pcDNA5/FRT/TO-GAlphaGustducin-RLuc8

    Plasmid
    #140979
    Purpose
    Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaGustducin-RLuc8 (GNAT3 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140979-plasmid-map-sequence-id-300662

    Enlarge

  12. pcDNA5/FRT/TO-GAlphaGustducin-RLuc8

    Plasmid
    #140979
    Purpose
    Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaGustducin-RLuc8 (GNAT3 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140979-plasmid-map-sequence-id-300662

    Enlarge

  15. pelB-MBP-HtrA-ss

    Plasmid
    #137066
    Purpose
    E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature HtrA: lacks the HtrA signal peptide
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137066-plasmid-map-sequence-id-291956

    Enlarge

  16. pelB-MBP-HtrA-ss

    Plasmid
    #137066
    Purpose
    E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature HtrA: lacks the HtrA signal peptide
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137066-plasmid-map-sequence-id-291956

    Enlarge

Showing: 2241 - 2260 of 20002 results