We narrowed to 15,156 results for: pET

1. pET28b-trAtCCD8(pYL736)

   Plasmid

   #178287

   Purpose

   Expresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   trAtCCD8
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a His6Flag2AUF1(p42)

   Plasmid

   #135941

   Purpose

   Expresses AUF1p42 in E. coli

   Depositor

   Yimon Aye

   Article

   Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

   Insert

   heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
   

   Tags

   Flag2 and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSHDY*in_PpetE:BCD2:mVenus

   Plasmid

   #133970

   Purpose

   Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.

   Depositor

   Pia Lindberg

   Article

   Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

   Insert

   mVenus
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   inserted gene is codon optimized for Synechocyst…

   Promoter

   PpetE:BCD2

   Available Since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-At_PRORP1-His

   Plasmid

   #67869

   Purpose

   bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

   Depositor

   Walter Rossmanith

   Article

   Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

   Insert

   PRORP1 (PRORP1 Mustard Weed)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Residues 36-572 (see comments)

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7CFE1-TelR15-YPet

   Plasmid

   #49646

   Purpose

   TALEYellow-Telomere for In Vitro Translation

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

   Insert

   TelR15
   

   Use

   In vitro translation

   Tags

   YPet

   Available Since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a(+)-HMGS

   Plasmid

   #108945

   Purpose

   heterologous expression of HMGS of E. faecalis in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   HMG-CoA synthase (HMGS)
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   A110G

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-PesaR-Plux

   Plasmid

   #47659

   Purpose

   PesaR-P promoter, luxCDABE, and terminator in pET17b (AmpR)

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Synth Biol. 2013 Jul 23.

   Insert

   PesaR-P
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Additional esa box downstream of the original esa…

   Promoter

   PesaR-P

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pET32a-His-FADD

   Plasmid

   #78803

   Purpose

   To purify His-tagged recombinant FADD protein

   Depositor

   Jaewhan Song

   Insert

   GIG3 (FADD Human)
   

   Tags

   6x His, S-Tag, Thrombin cleavage site, and TrxA (…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pET28a-ClbN-NHis

   Plasmid

   #51497

   Purpose

   NRPS module containing CATE domains with N-terminal 6His tag

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbN
   

   Tags

   6x His tag and T7 tag

   Expression

   Bacterial

   Mutation

   contains aa30-1094 of NP_754347.1

   Promoter

   T7

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-PTBP1-RRM12CCtoSS

    Plasmid

    #89155

    Purpose

    Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
    

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-ZZ(1700-1751)

    Plasmid

    #99334

    Purpose

    Bacterial expression of mouse CBP ZZ domain

    Depositor

    Peter Wright

    Article

    Legge et al J Mol Biol. 2004 Oct 29;343(4):1081-93.

    Insert

    CBP ZZ domain (Crebbp Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. ymScarletI-YPET pDRF1-GW

    Plasmid

    #168063

    Purpose

    FRET fusion between ymScarletI and YPET for use in yeast

    Depositor

    Bas Teusink

    Insert

    ymScarletI-YPET
    

    Expression

    Yeast

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-Cdc12-Cdc11

    Plasmid

    #131162

    Purpose

    Expression of Septin rods

    Depositor

    Nils Johnsson

    Article

    Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

    Insert

    6his-Cdc12 + Cdc11
    

    Use

    Bicistronic

    Tags

    Cdc11: S-Tag and Cdc12: 6his

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21-pelB-LaG-2

    Plasmid

    #172739

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-2
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-p53 (1-73) (72R)

    Plasmid

    #62082

    Purpose

    Expression of human p53 (1-73) (72R) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72R

    Promoter

    T7

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET11a-p52(1-398)

    Plasmid

    #44733

    Depositor

    Gourisankar Ghosh

    Insert

    p52 (NFKB2 Human)
    

    Expression

    Bacterial

    Mutation

    AA 1-398

    Promoter

    T7

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pET41s-GST-ATF6a-AD

    Plasmid

    #64774

    Purpose

    bacterial expression of GST-tagged ATF6 (1-150)

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    ATF6a-AD (1-150) (ATF6 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-term deletion

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET28a-Hook3 aa 1-239

    Plasmid

    #74609

    Purpose

    Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239 (HOOK3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET - FnR062.Y78W

    Plasmid

    #84802

    Purpose

    bacterial expression of FnR.0.6.2-Y78W protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78W
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78W

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

20. pET - FnR062.Y78D

    Plasmid

    #84786

    Purpose

    bacterial expression of FnR.0.6.2-Y78D protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78D
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78D

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only