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pCAGGS_cyt-AKAR5

Plasmid

#114138

Purpose

Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

AKAR5 with C-terminal nuclear export signal (NES)

Expression

Mammalian

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-Cα(S11A)-mPAGFP

Plasmid

#114134

Purpose

Mammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-CαN-mPAGFP

Plasmid

#114114

Purpose

Mammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP2B-siRNAres

Plasmid

#115333

Purpose

expresses siRNA-resistant mCherry-CHMP2B in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2B (CHMP2B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

mutated to be resistant to siRNA knockdown

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 R290A D291A T292A

Plasmid

#112103

Purpose

Mammalian expression of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R290A D291A T292A

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 D406A M407A

Plasmid

#112107

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D406A M407A

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 D406A M407A F409A

Plasmid

#112108

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D406A M407A F409A

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 D406R M407R

Plasmid

#112109

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D406R M407R

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 D406R M407R F409A

Plasmid

#112110

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D406R M407R F409A

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP

Plasmid

#65846

Purpose

Mammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Transmembrane (23 AA) of UQCC3 (UQCC3 Human)

Tags

GFP

Expression

Mammalian

Mutation

R5A-K6A

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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