We narrowed to 152,820 results for: Des

1. Tol2-LyzC-GFP-U6ac-cdk2-guides

   Plasmid

   #168250

   Purpose

   "neutrophil specific GFP with ubiquitous cdk2 sgRNAs"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   cdk2 sgRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Tol2-LyzC-RacFRET-U6ac-rac2 guides

   Plasmid

   #168248

   Purpose

   "label active Rac under Rac2 neutrophil-specific knockout background"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   RacFRET
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST

   Plasmid

   #189562

   Purpose

   Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

   Depositor

   Haian Fu

   Insert

   truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   contains aa 1-213

   Promoter

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

   Plasmid

   #69219

   Purpose

   Expresses wild type SpCas9 fused to Zif268 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   Zif268 (Egr1 Mouse)
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

   Plasmid

   #69227

   Purpose

   Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS4 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti CMV Puro DEST (w118-1) RALB

   Plasmid

   #107506

   Purpose

   Expressing RALB, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   RALB (RALB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PcDNA-DEST53-CypB-Golgi N-term GFP

   Plasmid

   #36127

   Depositor

   Patrick Van Oostveldt

   Article

   Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

   Insert

   cyclophilin B (PPIB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains amino acids 1-211 of cyclophilin B

   Promoter

   CMV

   Available since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

8. PcDNA-DEST47-CypB-Iso C-term GFP

   Plasmid

   #36130

   Depositor

   Patrick Van Oostveldt

   Article

   Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

   Insert

   cyclophilin B (PPIB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains amino acids 47-204 of cyclophilin B

   Promoter

   CMV

   Available since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

9. PcDNA-DEST47-CypB-Golgi C-term GFP

   Plasmid

   #36128

   Depositor

   Patrick Van Oostveldt

   Article

   Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

   Insert

   cyclophilin B (PPIB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains amino acids 1-211 of cyclophilin B

   Promoter

   CMV

   Available since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pDEST-FRT/T0-GFP-BRCA1 1364-1437

    Plasmid

    #71119

    Purpose

    Mammalian expression of GFP-BRCA1 1364-1437

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains amino acids 1364-1437 of BRCA1

    Promoter

    CMV/TO

    Available since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pLenti CMV Puro DEST (w118-1) CRIP2

    Plasmid

    #107509

    Purpose

    Expressing CRIP2, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    CRIP2 (codon optimized) (CRIP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized sequence

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PcDNA-DEST47-CypB-Nucl2 C-term GFP

    Plasmid

    #36126

    Depositor

    Patrick Van Oostveldt

    Article

    Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

    Insert

    cyclophilin B (PPIB Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains CypB AA 34-211

    Promoter

    CMV

    Available since

    Nov. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A

    Plasmid

    #51249

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    amino acids 980,1006 mutated to Ala

    Promoter

    EF1a

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

14. PcDNA-DEST47-CypB-Nucl C-term GFP

    Plasmid

    #36124

    Depositor

    Patrick Van Oostveldt

    Article

    Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

    Insert

    Cyclophilin B (PPIB Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains amino acids 34-216 of cyclophilin B

    Promoter

    CMV

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

15. PcDNA-DEST47-CypB-Del2 C-term GFP

    Plasmid

    #36134

    Depositor

    Patrick Van Oostveldt

    Article

    Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

    Insert

    cyclophilin B (PPIB Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains amino acids 38-216 of cyclophilin B

    Promoter

    CMV

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pLenti CMV Puro DEST (w118-1) FITM2

    Plasmid

    #107504

    Purpose

    Expresses FITM2, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    FITM2 (codon optimized)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized sequence

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PcDNA-DEST47-CypB-Del3 C-term GFP

    Plasmid

    #36136

    Depositor

    Patrick Van Oostveldt

    Article

    Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

    Insert

    cyclophilin B (PPIB Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains amino acids 39-216 of cyclophilin B; G63…

    Promoter

    CMV

    Available since

    Nov. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

18. PcDNA-DEST47-CypB-Del1 C-term GFP

    Plasmid

    #36132

    Depositor

    Patrick Van Oostveldt

    Article

    Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

    Insert

    cyclophilin B (PPIB Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains amino acids 37-216 of cyclophilin B

    Promoter

    CMV

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2

    Plasmid

    #69225

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS2 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A

    Plasmid

    #51247

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    amino acids 849,865,877,907 mutated to Ala

    Promoter

    EF1a

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only