We narrowed to 3,949 results for: SPL
-
Plasmid#68438PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U1Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pEF1a-DIO-tTAc
Plasmid#172126PurposeExpression of InteinC-tTAC in Cre recombinase positive cellsDepositorInsertInteinC-tTAc
UseAAVTagsExpressionMutationPromoterCaMKIIAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FRT-EBFP-tTAn
Plasmid#172127PurposeExpression of EBFP and tTA-InteinN in Flp recombinase positive cellsDepositorInsertEBFP, tTAn-InteinN
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP1-10 mitochondrial matrix
Plasmid#172063PurposeUAS vector to express GFP1-10 in the mitochondrial matrixDepositorInsertGFP1-10 mitochondrial matrix
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX601‐mhCMV‐ABEmaxNGA‐C3‐ E53ogRNA
Plasmid#187063PurposeNpu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutationDepositorInsertNpu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermhCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[P4-P6(3xPP7)]
Plasmid#68432PurposeTransient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pmyo3::tag:sl1(G16C)
Plasmid#79003PurposeExpression of exogenous SL1 in C. elegans muscleInsertsls-1.2 (sls-1.2 Nematode)
UseTagsillumina-adapterExpressionWormMutationG16CPromotermyo-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-MyD88 (S34Y)-CpLxIS
Plasmid#131349PurposeExpresses MyD88 S34Y with 2 copies of pLxIS motif from STING fused to its C-terminusDepositorInsertMyD88 (S34Y)-CpLxIS (Myd88 Mouse)
UseRetroviralTagsExpressionMammalianMutationMyD88 (S34Y)PromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRCHisB-TRF2_M
Plasmid#50490PurposeE. Coli N-term HexHis Tagged Protein Expression VectorDepositorInsertTRF2 Myb (TERF2 Human)
UseTags6x His, T7 tag, and TEV protease siteExpressionBacterialMutationContains amino acids 438-500PromoterpTrcAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
NSSCP
Plasmid#183190PurposemRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEVDepositorInsertsmRFP-PopZ
DivIVA-GFP(tevR-50)
SpmX-TEV(C)
TEV(N)-SpmX
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Desmin/pAS2-1
Plasmid#97407PurposeDesmin (aa 30 – 103) in DNA-binding domain (BD) vector, used for yeast-two hybridDepositorInsertDesmin (DES Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD-BirA-eCPX-AP(K10)
Plasmid#121908PurposeExpresses BirA and eCPX-AP(K10)DepositorInsertsUseTags6xHisExpressionBacterialMutationchanged lysine 10 in the AP to alaninePromoterT7 and pBAD promoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM2-hPMCA2x/b
Plasmid#47582DepositorInsertPMCA2x/b (ATP2B2 Human)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
SFG.CNa22_opt.IRES.eGFP
Plasmid#22491DepositorInsertCodon optimised Calcineurin A mutant 22 (PPP3CA Human)
UseRetroviralTagsExpressionMutationV314R; Y341FPromoterAvailable sinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
IBMc097
Plasmid#161945PurposeContains Type IIS restriction site free elements for IBM staging vectors. Staging plasmid for IBM on mCherryDepositorInsertpSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)MBII-85-5'/3'ss mut
Plasmid#67645PurposeMBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.DepositorInsertMBII85 snoRNA
UseTagsExpressionMammalianMutation5' and 3' splice sites mutationsPromoterAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pmyo-3::tag:SL1(G20A)
Plasmid#81114PurposeExpression of exogenous SL1(G20A) in C. elegans muscleDepositorInsertsls-1.2 (sls-1.2 Nematode)
UseTagsillumina-adapterExpressionWormMutationG20APromoterpromoter of myo-3Available sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MA-VN
Plasmid#123283PurposeMammalian expression plasmid for matrix domain of HIV-1 Gag fused to the N-terminal half of split fluorescent Venus (VN)DepositorInsertMatrix
UseTagsVN: N-terminus of split Venus (a.a. V1–A154) I152…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLSU4mg10
Plasmid#126079PurposePlant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cellsDepositorInsertmitochondria Rieske iron sulphur protein (1-100 aa)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoter'long' CaMV35SAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1388 TDP43 RRM-GFP K-R
Plasmid#118796PurposeTo test the effect of sequence on TDP43 RRM-GFP droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll K to R in CTD of TDP43 RRM-GFP reporterPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only