We narrowed to 8,441 results for: poly

1. pcDNA3-CD16.7-hPC1-eGFP-3HA

   Plasmid

   #108401

   Purpose

   Express cytoplasmic tail human PC1 from amino acid 4077 to 4302 with a membrane anchor

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Human)
   

   Use

   Tags

   eGFP-3HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Control click editor - pCMV-T7-PCV2-nCas9-deadEcKlenow (JO520)

   Plasmid

   #208945

   Purpose

   A control CE1 construct with catalytically attenuated EcKlenow DNA polymerase, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-dEcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A);deadEcKlenow(-exo;D355A,D357A,D705…

   Promoter

   CMV and T7

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-hPC1_MTS_L4139P-eGFP-3HA

   Plasmid

   #108404

   Purpose

   Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Human)
   

   Use

   Tags

   3xHA and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-mPC1_4119-eGFP

   Plasmid

   #108394

   Purpose

   Genomic region, including intron 45, to express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   variant ID rs13468401 missense variant R4237Q

   Promoter

   CMV

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-mPC1_4148-3HA

   Plasmid

   #108398

   Purpose

   Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   Pkd1 (Pkd1 Mouse)
   

   Use

   Tags

   3HA

   Expression

   Mammalian

   Mutation

   variant ID rs13468401 missense variant R4237Q

   Promoter

   CMV

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-mPC1_4148-eGFP

   Plasmid

   #108397

   Purpose

   Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   Pkd1 (Pkd1 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   variant ID rs13468401 missense variant R4237Q

   Promoter

   CMV

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-mPC1_4148-eGFP-3HA

   Plasmid

   #108396

   Purpose

   Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   Pkd1 (Pkd1 Mouse)
   

   Use

   Tags

   eGFP-3HA

   Expression

   Mammalian

   Mutation

   variant ID rs13468401 missense variant R4237Q

   Promoter

   CMV

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-hPC1_MTS_V4146I-eGFP

   Plasmid

   #108405

   Purpose

   Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely non-pathogenic variant.

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Mouse)
   

   Use

   Tags

   3xHA and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-hPC1_MTS_L4137P-eGFP

   Plasmid

   #108403

   Purpose

   Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Mouse)
   

   Use

   Tags

   3xHA and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-hPC1_MTS-eGFP

    Plasmid

    #108402

    Purpose

    Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Mouse)
    

    Use

    Tags

    3xHA and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-mEtfdh

    Plasmid

    #232314

    Purpose

    Expression of mouse mutant Etfdh

    Depositor

    Ivan Topisirovic

    Article

    Papadopoli et al bioRxiv 2024.10.25.620155

    Insert

    Etfdh (Etfdh Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    PAM Sequence Mutations: (G36C, T39C, C42T, C72T, …

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWZL Neo Myr Flag PIK4CA

    Plasmid

    #20577

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PIK4CA (PI4KA Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pWZL Neo Myr Flag RPSK6A3

    Plasmid

    #20627

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    RPSK6A3 (RPS6KA3 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pWZL Neo Myr Flag PIK3CG

    Plasmid

    #20574

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PIK3CG (PIK3CG Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pYeDP60-hATP13A2-E343A

    Plasmid

    #171378

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Use

    Tags

    BAD tag

    Expression

    Yeast

    Mutation

    E343A

    Promoter

    URA3

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWZL Neo Myr Flag PIK3CB

    Plasmid

    #20573

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PIK3CB (PIK3CB Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWZL Neo Myr Flag RPS6KA5

    Plasmid

    #20622

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    RPS6KA5 (RPS6KA5 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pFBOH-MHL.EED

    Plasmid

    #125174

    Purpose

    Expresses human EED in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    Polycomb protein EED (EED Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)

    Plasmid

    #169185

    Purpose

    Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    His6-3xFlag (nsp12 C-terminus)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid

    #169180

    Purpose

    Baculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp7-His6-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    His6 (internal tag linking nsp7 and nsp8): nsp7-H…

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge