We narrowed to 4,671 results for: ado

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pPHR-mCherry-hNPM

Plasmid

#181887

Purpose

Soluble light-dependent effector protein (nucleophosmin) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-hNPM (NPM1 Human, Mustard Weed)

Tags

PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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JDW 762 (pCS2-EGFP-hsKRAS4B-G12V)

Plasmid

#156407

Purpose

pCS2 CMV expression vector driving EGFP fused human KRAS4B-G12V

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4B-G12V (KRAS Human)

Tags

EGFP

Expression

Mammalian

Mutation

mutant KRAS4B-G12V

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald D40G

Plasmid

#164211

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Mutation

D40G

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter

Plasmid

#172597

Purpose

Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.

Depositor

Daniel Kiss

Article

Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

Insert

myc-PDGFRA-K627R (PDGFRA Synthetic, Human)

Use

Egfp-pest_reporter

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

PDGFRA-K627R

Promoter

CMV

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald R346C

Plasmid

#164213

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R346C mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Mutation

R346C

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald R235Q

Plasmid

#164212

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Mutation

R235Q

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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EFSp-GFP-YAP (S94A)

Plasmid

#174169

Purpose

Lentiviral vector to constitutively express TEAD-binding mutant YAP under control of the EFS promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Mutation

Serine 94 mutated to Alanine

Promoter

EFS

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CMV-CRY2oligo-mcherry-ANXA11(R235Q)

Plasmid

#164205

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 R235R mutant under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

R235Q

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-CRY2oligo-mcherry-ANXA11(D40G)

Plasmid

#164204

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 D40G mutant under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

D40G

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-1BPNLS-Cas9-1BPNLS-2AGFP

Plasmid

#87109

Purpose

BPNLS-Cas9-BPNLS-2AGFP expression plasmid

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

1BPNLS-Cas9-1BPNLS-2A-EGFP

Use

CRISPR

Tags

3xFLAG (on Cas9)

Expression

Mammalian

Promoter

CAG

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_38

Plasmid

#60279

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ACSL1 enhancer (ACSL1 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pET28 His-MBP-TEV-BAP-Human BCKDK

Plasmid

#232116

Purpose

For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.

Depositor

Ruth Sommese

Article

Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

Insert

BCKDK (BCKDK Human)

Tags

6 x His, Biotin acceptor peptide (BAP), Maltose-B…

Expression

Bacterial

Promoter

T7 Promoter

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 742 (pDONR221-EGFP-KRAS4B-G12V)

Plasmid

#156403

Purpose

Gateway middle entry clone encoding EGFP-fused to human KRAS4B with G12V mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4B-G12V (KRAS Human)

Use

Gateway cloning

Tags

EGFP

Mutation

mutant KRAS4B-G12V

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

Plasmid

#171802

Purpose

Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mNeonGreen-FLAG-mgp100-T2A-PuroR

Use

Gene tagging

Mutation

PuroR: Changed Methionin 1 to Glycine.

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-hHP1gamma

Plasmid

#181885

Purpose

Soluble light-dependent effector protein (human HP1gamma) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-hHP1gamma (CBX3 Human, Mustard Weed)

Tags

PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-mHP1alpha

Plasmid

#181883

Purpose

Soluble light-dependent effector protein (mouse HP1alpha) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-mHP1alpha (Cbx5 Mouse, Mustard Weed)

Tags

PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_33

Plasmid

#60275

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

GLIS3 enhancer (GLIS3 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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JDW 837 (pCS2-V5-mScarlet-I-hsKRAS4A-G12V)

Plasmid

#156410

Purpose

pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A-G12V

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12V (KRAS Human)

Tags

mScarlet-I

Expression

Mammalian

Mutation

mutant KRAS4A-G12V

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)

Plasmid

#156409

Purpose

pCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12D

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12D (KRAS Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

mutant KRAS4A-G12D

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_13

Plasmid

#60298

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

EDN3 enhancer (EDN3 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only