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pDREV-0

Plasmid

#26750

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Insert

H2B-Venus CDS (H2BC3 Human)

Use

Cre/Lox; Flp/frt; dre/rox

Tags

Venus

Expression

Mammalian

Available Since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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lentiguide-puro-wt-ltr

Plasmid

#182635

Purpose

Expresses guide RNA, contains intact LTR

Depositor

Jakob Reiser

Article

Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

Insert

S. pyogenes sgRNA cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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lenticrispr-wt-ltr-puro

Plasmid

#173428

Purpose

Expresses Cas9 and guide RNA, contains intact LTR

Depositor

Jakob Reiser

Article

Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

Insert

S. pyogenes sgRNA cassette

Use

Lentiviral

Expression

Mammalian

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-17

Plasmid

#119996

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mEos4b

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mEos4b

Use

Pcr template

Tags

mEos4b

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-kanaR-cryaa-YFP

Plasmid

#74154

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Citrine

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-YFP

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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MSGV Rh Acceptor

Plasmid

#64271

Purpose

Retroviral golden gate cloning vector for expression of functional Rhesus Macaque T-Cell Receptor genes

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-IndHom-Tol2-FRT-GalK-cryaa-dsRed

Plasmid

#73203

Purpose

BAC targeting cassette for pIndigoBAC-536 with GalK selection marker and lens-specific transgenesis marker dsRed

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

dsRed

Available Since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pSHS248

Plasmid

#87354

Purpose

S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S/S867C/N1054C

Promoter

T7

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMC-1-35S_Long-6

Plasmid

#197698

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

35S promoter (1.3kb)

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-34

Plasmid

#119998

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-FRT-GalK-cryaa-CFP

Plasmid

#73200

Purpose

BAC targeting cassette for pTARBAC2 with GalK selection marker and lens-specific transgenesis marker Cerulean

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

CFP

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pFLIP38

Plasmid

#65616

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-edCit-attR1 and attR2-edCer-cMyc

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pMC-1-DR5v2-4

Plasmid

#197695

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

DR5v2 promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-27

Plasmid

#120006

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 1xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

1xHA

Use

Pcr template

Tags

1xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMC-1-OsEf1a-6

Plasmid

#197703

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

OsEf1a promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-OsUbi-6

Plasmid

#197704

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

OsUbi promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-ZmEf1a-6

Plasmid

#197712

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmEf1a promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-OsEf1a-11

Plasmid

#197766

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

OsEf1a terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-OsT28-11

Plasmid

#197767

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

OsT28 terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-OsUbi-11

Plasmid

#197768

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

OsUbi terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-ZmEf1a-11

Plasmid

#197775

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmEf1a terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-ZmIn2-11

Plasmid

#197776

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Maize In2-1 (Saf1 locus) terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-FLuc-9

Plasmid

#197737

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Firefly luciferase

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-AtHSP-11

Plasmid

#197760

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AtHSP terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAIO-AsCpf1

Plasmid

#137846

Purpose

AsCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

AsCpf1-NLS-FLAG

Use

CRISPR

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDGB1_omega1R

Plasmid

#68236

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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YeNL-H2B_pcDNA3

Plasmid

#89522

Purpose

Yellow color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Tags

histone 2B

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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SPDK3214

Plasmid

#97406

Purpose

binary construct of ER-targeted GUS with sfCherry11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

ER-GUS-sfCherry11

Tags

ER target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-FRT-GalK-cryaa-YFP

Plasmid

#73198

Purpose

BAC targeting cassette for pTARBAC2 with GalK selection marker and lens-specific transgenesis marker Citrine

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

YFP

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLIP43

Plasmid

#65618

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-edAFPt9-attR1 and attR2-edCer-cMyc

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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