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pGST-POLA1-N2

Plasmid

#160985

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N1

Plasmid

#160984

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNArodA

Plasmid

#149655

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PresTS-sgRNAflaB

Plasmid

#149644

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, PresTS

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PresTL-sgRNAflaB

Plasmid

#149645

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, PresTL

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0026-sgRNAflaB

Plasmid

#149646

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0026

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0826S-sgRNAflaB

Plasmid

#149647

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0826S

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAftsI

Plasmid

#149649

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNAftsI

Plasmid

#149650

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNAftsI

Plasmid

#149563

Purpose

ftsI gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNAmreB

Plasmid

#149567

Purpose

mreB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1052B (Ubiq-Fluc_Ubiq-Rluc)

Plasmid

#132426

Purpose

Express Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Inserts

Ubiq promoter
firefly luciferase
T2A-eGFP
Renilla Luciferase-SV40-P10
reversed ubiq promoter

Expression

Insect

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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Y365A-GFP Donor for iMTr EED

Plasmid

#131325

Purpose

Donor to generate i-MT-r system

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

i-MT-r.Donor

Use

CRISPR and Mouse Targeting

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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A365Y-GFP Donor for iWTr EED

Plasmid

#131324

Purpose

Donor to generate i-WT-r system

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

i-WT-r.Donor

Use

CRISPR and Mouse Targeting

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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EED-KO-gRNA-2

Plasmid

#131322

Purpose

gRNA to knockout EED. Use with EED-KO-gRNA-1

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EED-KO-gRNA-2

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
pJM161

Plasmid

#134170

Purpose

Retroviral plasmid expressing GFP Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC 318AAA320 (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC 318AAA320

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJM134

Plasmid

#134165

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_KMLL 1-330 (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

KMLL 1-330

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJM132

Plasmid

#134164

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_KMLL 1-320 (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

KMLL 1-320

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM131

Plasmid

#134163

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM110

Plasmid

#134161

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC 290-C (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM011

Plasmid

#134155

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC 266-C (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC 266-C

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM008

Plasmid

#134153

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1 1-265 (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

1-265

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM007

Plasmid

#134152

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1 1-283 (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

1-283

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM006

Plasmid

#134151

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1 1-316 (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

1-316

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3

Plasmid

#133343

Purpose

for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_gcrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA

Plasmid

#133345

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_blaA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMET17p-CCG 316

Plasmid

#131166

Purpose

For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

Type

Empty backbone

Tags

CCG

Expression

Yeast

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Vmn2r65

Plasmid

#108083

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r65 gene

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r65 (Vmn2r65 Mouse)

Use

In vitro transcription

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Vmn2r76-VR2

Plasmid

#108085

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r76 gene

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r76 (Vmn2r76 Mouse)

Use

In vitro transcription

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Vmn2r118

Plasmid

#108086

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r118 gene

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r118 (Vmn2r118 Mouse)

Use

In vitro transcription

Mutation

changed T to C at nt 830 of NM_001104582.1

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Vmn2r120-TR

Plasmid

#108087

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r120 gene

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r120 (Vmn2r120 Mouse)

Use

In vitro transcription

Mutation

changed A to G at nt 1196 of NM_001104591.1

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gh144

Plasmid

#106803

Purpose

expression of gRNA targeting LARGE

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

LARGE

Expression

Mammalian

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gh119

Plasmid

#106789

Purpose

expression of gRNA targeting GCNT6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GCNT6

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gh20

Plasmid

#106704

Purpose

expression of gRNA targeting GALNT20

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT20

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MRLC-DD-pIRES-EGFP-AP4-mito

Plasmid

#101287

Purpose

To define the role of Mena/VASP in tension-sensitive actin assembly

Depositor

Alpha Yap

Article

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

MRLC-DD and AP4-Mito

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ELK1 A box-NLS pcDNA3.1/HisC

Plasmid

#121096

Purpose

Expression of the A box fragment of ELK1

Depositor

Paul Herring

Article

Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

Insert

ELK1 (Elk1 Mouse)

Tags

6XHis-Omni-xpress

Expression

Mammalian

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ELK1 B box-NLS pcDNA3.1/HisC

Plasmid

#121095

Purpose

Expression of the B box fragment of ELK1

Depositor

Paul Herring

Article

Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

Insert

ELK1 (Elk1 Mouse)

Tags

6XHis-Omni-xpress

Expression

Mammalian

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gh163

Plasmid

#106816

Purpose

expression of gRNA targeting TMTC4

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

TMTC4 (TMTC4 Human)

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gh41

Plasmid

#106715

Purpose

expression of gRNA targeting ST6GAL2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST6GAL2 (ST6GAL2 Human)

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gh156

Plasmid

#106823

Purpose

expression of gRNA targeting GYLT1B (LARGE2)

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GYLT1B (LARGE2)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSO9

Plasmid

#106366

Purpose

To insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the intestine.

Depositor

Jonathan Ewbank

Article

Lee et al Virulence. 2018 Dec 31;9(1):648-658. doi

Insert

ttTi4348_ SEC _pvit-6_OspF_3'unc54

Expression

Worm

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKB3SaGGR I206F (JBEI-3678)

Plasmid

#110149

Purpose

SaGGR site-directed mutation of I206F

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Expression

Bacterial

Mutation

SaGGR site-directed mutation of I206F

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKB3SaGGR G91H (JBEI-3677)

Plasmid

#110148

Purpose

SaGGR site-directed mutation of G91H

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Expression

Bacterial

Mutation

SaGGR site-directed mutation of G91H

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKB3SaGGR L377H (JBEI-3683)

Plasmid

#110150

Purpose

SaGGR site-directed mutation of L377H

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Expression

Bacterial

Mutation

SaGGR site-directed mutation of L377H

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Drd2

Plasmid

#105482

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Drd2 (Drd2 Mouse)

Use

To ganerate riboprobe

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRRNA-G11-R0

Plasmid

#101805

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Promoter

Spy 1049

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-Metavinculin tail

Plasmid

#107152

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al Proc Natl Acad Sci U S A. 2016 Aug 23;11

Insert

metavinculin (VCL Human)

Tags

(His)8

Expression

Bacterial

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRRNA-G0-R11

Plasmid

#101808

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Promoter

Spy 1049

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRRNA-G0-R18

Plasmid

#101813

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Promoter

Spy 1049

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRRNA-G14-R0

Plasmid

#101806

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Promoter

Spy 1049

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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