We narrowed to 7,363 results for: 11

1. pIntegrase_5

   Plasmid

   #60577

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_5
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPLtetO-ClpX

   Plasmid

   #89657

   Purpose

   Plasmid for titrating synthesis of ClpX with doxycycline

   Depositor

   Uwe Sauer

   Article

   Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

   Insert

   clpX
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNAS1B split mCherry N-link/link-C

   Plasmid

   #61965

   Purpose

   Negative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)

   Depositor

   Lynne Regan

   Article

   Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

   Inserts

   N-mCherry-link
   link-C-mCherry
   

   Expression

   Bacterial

   Promoter

   PBAD and PLtetO

   Available Since

   Feb. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. EYFP-Clathrin

   Plasmid

   #20921

   Depositor

   Xiaowei Zhuang

   Article

   Rust et al Nat Struct Mol Biol. 2004 Jun . 11(6):56

   Insert

   Clathrin, light polypeptide (Lca) (Clta Mouse)
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

5. RP1B-PP1alpha

   Plasmid

   #51768

   Purpose

   Expresses PP1 alpha residues 7-330

   Depositor

   Wolfgang Peti

   Article

   Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

   Insert

   pp1 alpha
   

   Tags

   His, TEV protease site, and Thio-6

   Expression

   Bacterial

   Mutation

   Contains PP1 alpha residues 7-330

   Promoter

   T7-lac

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pMSC236

   Plasmid

   #216368

   Purpose

   xhNup155-Nb2i (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup155-Nb2i (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSC235

   Plasmid

   #216372

   Purpose

   xhNup155-Nb3i (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup155-Nb3i (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSC227

   Plasmid

   #216351

   Purpose

   xhNup35-Nb1t (+3Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup35-Nb1t (+3Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 Flag FKHR H215R

   Plasmid

   #13509

   Depositor

   Kunliang Guan

   Article

   Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

   Insert

   FKHR H215R (FOXO1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   H215R

   Available Since

   Dec. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pCas_5+7_gfp

    Plasmid

    #60595

    Purpose

    encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Cas_5+7_gfp
    

    Expression

    Bacterial

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS_FLPER(T2)

    Plasmid

    #31303

    Depositor

    Gerhart Ryffel

    Article

    Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

    Insert

    FLP-ER ligand binding domain (ESR1 Human, Budding Yeast)
    

    Expression

    Mammalian

    Mutation

    full length FLP linked to ligand binding domain o…

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPBO.ACT005

    Plasmid

    #182715

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBlastR-12xLexAOp-mCherry-MiniWhite

    Plasmid

    #165917

    Purpose

    Blasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable LexAOp Response Vector
    

    Use

    Synthetic Biology

    Available Since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLSU4cg10

    Plasmid

    #126078

    Purpose

    Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    Chloroplastic FNR transit peptide
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Promoter

    longCaMV35S

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSG424 Smad2 (Gal4-Smad2)

    Plasmid

    #14932

    Depositor

    Joan Massague

    Article

    Liu et al Genes Dev. 1997 Dec 1. 11(23):3157-67.

    Insert

    Smad2 (SMAD2 Human)
    

    Tags

    Gal4

    Expression

    Mammalian

    Available Since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pCFJ1272

    Plasmid

    #44486

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, MCS
    

    Use

    Multiple cloning site vector

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIntegrase_12

    Plasmid

    #60583

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_12
    

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-nDsRedFL-miR9/9*

    Plasmid

    #177805

    Purpose

    The pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-9/9*
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSC130

    Plasmid

    #216364

    Purpose

    xhNup98-Nb3i Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup98-Nb3i
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTAJAK-71 (pESC-NatMXsyn-USER)

    Plasmid

    #78232

    Purpose

    Empty vector, for the insertion of gRNA expression cassettes into the vector.

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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