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pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31

Plasmid

#165889

Purpose

Blasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

All-in-one FC31 RMCE Cassette

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGal4BD 1-147

Plasmid

#165819

Purpose

1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Gal4BD 1-147

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL4AD 768-881

Plasmid

#165820

Purpose

768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GAL4AD 768-881

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Vimentin-DiB-RM

Plasmid

#168783

Purpose

Vimentin - DiB-RM fusion protein

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

Vimentin-DiB-RM

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGMR-WhiteA2

Plasmid

#165874

Purpose

Basic transgenesis 5xGMR white CDS physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPuroR-GMRWhite

Plasmid

#165884

Purpose

Puromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Transgenesis Ready Selection Vector

Use

Synthetic Biology

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pU2T2U2T2-YFP

Plasmid

#84550

Purpose

Hybrid reporter construct with 8 transcription factor binding sites (2 UAS, 2 TetO, 2 UAS, 2 TetO) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T2U2T2-EYFP

Expression

Mammalian

Promoter

CMV-min

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT2U2T2U2-YFP

Plasmid

#84552

Purpose

Hybrid reporter construct with 8 transcription factor binding sites (2 TetO, 2 UAS, 2 TetO, 2 UAS) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U2T2U2-EYFP

Expression

Mammalian

Promoter

CMV-min

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Rap-MESA-20-Tev

Plasmid

#84555

Purpose

MESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev protease

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

Rap-MESA-FKBP-20-Tev

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFLinkC-AI(G3)-PDZ(GPG-G)FN3(GGS4)-TVMV

Plasmid

#137117

Purpose

Protease Switch Clone ID: 2-A5

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

AI(G3)-PDZ(GPG-G)FN3(GGS4)-TVMV

Use

Synthetic Biology

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFLinkC-AI(GPG)-FKBP12(TP11)-FRB(GPG)-TVMV

Plasmid

#137115

Purpose

Protease Switch Clone ID: 1-A9

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

AI(GPG)-FKBP12(TP11)-FRB(GPG)-TVMV

Use

Synthetic Biology

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFLinkC-AI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMV

Plasmid

#137116

Purpose

Protease Switch Clone ID: 1-C9

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

AI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMV

Use

Synthetic Biology

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pEY31CBiT

Plasmid

#127761

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

Convergent LacI-TetR Toggle

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEYD1A

Plasmid

#127759

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach, MG RNA aptamer in divergent orientation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEYRC-D1A

Plasmid

#127753

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

RFP, CFP, Divergent Orientation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEYT1A

Plasmid

#127760

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach, MG RNA aptamer in tandem orientation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pEYRC-C1A

Plasmid

#127752

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

RFP, CFP, Convergent Orientation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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IgH P+ VA20

Plasmid

#127249

Purpose

IgH V-region

Depositor

Christine Milcarek

Article

Shell et al J Immunol. 2007 Dec 1;179(11):7663-73.

Insert

Promoter+V-region of IgH of A20 B cell line

Use

Other

Promoter

IgH

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA008

Plasmid

#128512

Purpose

Expresses Xylanase C

Depositor

Marcos Alcocer

Article

Furniss et al Biochim Biophys Acta. 2002 Jul 29;1598(1

Insert

Xylanase C

Expression

Bacterial

Promoter

Histone 4B

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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Hsh155 Hs 1-16 3x FLAG

Plasmid

#111966

Purpose

Human HEATs 1-16 have been inserted into Hsh155 3xFLAG.

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 Hs 1-16 3x FLAG

Tags

3xFLAG

Expression

Bacterial and Yeast

Mutation

Human HEATs 1-16 inserted

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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