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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 20,158 results for: Nov

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Showing: 2281 - 2300 of 20158 results

  2. CMV-enAsCas12f

    Plasmid
    #204637
    Purpose
    Expression of enAsCas12f in mammalian cells
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enAsCas12f
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    D196K, N199K, G276R, N328G, D364R
    Promoter
    CMV
    Available since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    204637-plasmid-map-sequence-id-403532

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  3. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

    Plasmid
    #62373
    Purpose
    Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells
    Depositor
    David Liu
    Publication
    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do
    Insert
    (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
    Tags
    6xHis tag, (-30)dGFP, Cathepsin cleavage site, an…
    Expression
    Bacterial
    Mutation
    Y67S amino acid substitution in (-30)GFP renders …
    Promoter
    T7
    Available since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    62373-plasmid-map-sequence-id-263816

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  7. CTGF-bio-His

    Plasmid
    #52028
    Purpose
    Expresses full-length Connective tissue growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CTGF (CCN2 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52028-plasmid-map-sequence-id-80102

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  12. pEGFPC1-hVAP-B KD/MD

    Plasmid
    #104450
    Purpose
    Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells
    Depositor
    Catherine Tomasetto
    Publication
    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    vesicle-associated membrane protein-associated protein B (VAPB Human)
    Tags
    EGFP and HA
    Expression
    Mammalian
    Mutation
    changed K87 to D and M89 to D
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104450-plasmid-map-sequence-id-201410

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  13. p5'Entry-lck-5.5kb

    Plasmid
    #58890
    Purpose
    provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes
    Depositor
    Jeffrey Yoder
    Insert
    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
    Use
    Gateway 5' entry clone
    Mutation
    mutated single nucleotide (T-->C, 13th nucleot…
    Promoter
    lymphocyte-specific protein tyrosine kinase (lck)…
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58890-plasmid-map-sequence-id-91281

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  14. pTriEx-PA-Rac1-C450A

    Plasmid
    #22025
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Publication
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    Tags
    6X His
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV domain contains light-insensitive mutation C4…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  16. Dead Streptavidin-SpyCatcher

    Plasmid
    #59547
    Purpose
    pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Publication
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead-dloop-Streptavidin-SpyCatcher
    Tags
    N-terminally shortened SpyCatcher
    Expression
    Bacterial
    Mutation
    PolyAsp in L3/4 loop for ion-exchange
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 2281 - 2300 of 20158 results