167,433 results

1. Flag-PML IV/pRK5

   Plasmid

   #59742

   Purpose

   Expresses the isoform IV of Promyelocytic Leukemia Protein (PML) in mammalian cells

   Depositor

   Xiaolu Yang

   Article

   Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

   Insert

   PML isoform IV (PML Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-PEmax-SpG

   Plasmid

   #211549

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

   Insert

   PEmax-SpG
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDT305

   Plasmid

   #128801

   Purpose

   Human codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone

   Depositor

   Bradley Bernstein

   Article

   Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

   Insert

   dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
   

   Use

   CRISPR and Lentiviral

   Promoter

   hUBC promoter

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-AML1/ETO-IRES-GFP

   Plasmid

   #60832

   Purpose

   AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

   Depositor

   Scott Lowe

   Article

   Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

   Insert

   AML1/ETO (RUNX1, RUNX1T1 Human)
   

   Use

   Retroviral

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLminP_Luc2P_RE5

   Plasmid

   #90339

   Purpose

   STAT3 - Stat3 homodimer gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   STAT3

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. V5-GFP-TurboID-KDEL_pLX208

   Plasmid

   #202020

   Purpose

   expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   V5-TurboID-KDEL
   

   Use

   Lentiviral

   Tags

   GFP and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tet-pLKO-puro-SOX2

   Plasmid

   #47540

   Purpose

   Tet inducible knockdown of SOX2.

   Depositor

   Charles Rudin

   Article

   Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

   Insert

   SOX2 shRNA
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. E-cadherin-GFP

   Plasmid

   #28009

   Depositor

   Jennifer Stow

   Article

   Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

   Insert

   E-cadherin (CDH1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. 8xNFAT-ZsG-hCD8

   Plasmid

   #153417

   Purpose

   NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

    Plasmid

    #223175

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Mutation

    DR1 A>T

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV1)

    Viral Prep

    #100854-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Promoter

    Syn

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pSLQ9663 - ACTB GFP-P2A CLIP Donor

    Plasmid

    #202688

    Purpose

    CLIP donor template for integrating GFP into ACTB locus. Encodes GFP with ACTB homology arms.

    Depositor

    Stanley Qi

    Article

    Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

    Insert

    CopGFP
    

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDD120

    Plasmid

    #119879

    Purpose

    Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pConstitutive_Che9c60 and Che9c61
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA5/FRT/TO_HaloTag7_T2A_EGFP

    Plasmid

    #169325

    Purpose

    Expression of HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-CMV-jGCaMP7f

    Plasmid

    #104483

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7f
    

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. 4xnrUAS:GFP

    Plasmid

    #61373

    Purpose

    4xnrUAS driving GFP

    Depositor

    Marnie Halpern

    Article

    Akitake et al Dev Biol. 2011 Apr 15;352(2):191-201. do

    Insert

    4xnr UAS:GFP
    

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-KIT-V5/HIS

    Plasmid

    #201986

    Purpose

    expression of human KIT receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    KIT receptor tyrosine kinase (KIT Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CD9-mEGFP

    Plasmid

    #182864

    Purpose

    Localization: Tetraspanin/Membrane

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    human CD9 (CD9 Human)
    

    Expression

    Mammalian

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. elavl3:hGCGR-TEVcs-QF;he1.1:CFP

    Plasmid

    #218852

    Purpose

    D. rerio elavl3 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCGR (GCGR Human)
    

    Tags

    QF fusion protein

    Expression

    Bacterial

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. tdTomato-anti-AlfaTag nanobody

    Plasmid

    #171819

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    tdTomato-anti-AlfaTag nanobody
    

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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