307,875 results

1. PKM2

   Plasmid

   #25538

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PKM2 (PKM Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 24, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pcDNA3-HA-MKRN1

   Plasmid

   #78745

   Purpose

   To overexpress MKRN1 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

   Insert

   MKRN1 (MKRN1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pICSL30008

   Plasmid

   #50300

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   N terminal HA tag (6x Human influenza hemagglutinin)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMpGWB106

   Plasmid

   #68560

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Citrine

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGGF_RT_FAST_Tq2CFP

   Plasmid

   #136981

   Purpose

   Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

   Depositor

   Klaus Theres

   Insert

   seed coat marker, replaced RFP w/ Tq2CFP. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

   Promoter

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGM34DEST

   Plasmid

   #74756

   Purpose

   Encodes for the 5X repeat of the QUAS response element, followed by the delta pes-10 minimal promoter

   Depositor

   Jordan Ward, Keith Yamamoto

   Article

   Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

   Insert

   Gateway cassette
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMIN-VS1

   Plasmid

   #197783

   Purpose

   Level 2 destination vector: pMIN-VS1 backbone

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 13XLexAop2 IVS ChrimsonR mCherry_tr

   Plasmid

   #111548

   Purpose

   Optogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherry

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR_mCherry_trafficked
   

   Use

   Tags

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP_sfCherry2(11)_zyxin

   Plasmid

   #83034

   Purpose

   Expresses sfCherry2(11) tagged zyxin (human) in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(11)_zyxin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223_FH_WT

    Plasmid

    #82210

    Purpose

    Gateway Donor vector containing FH , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    FH (FH Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pICSL30009

    Plasmid

    #50301

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    N terminal Myc tag (4x Myc)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFRT/TO/HIS/FLAG/HA-YTHDF2

    Plasmid

    #38089

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    YTHDF2 YTH domain family, member 2 (YTHDF2 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXS-IRES-Blast ACSL4

    Plasmid

    #173164

    Purpose

    Retroviral vector to express sgRNA resistant human ACSL4

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5-FRT-TetO-U1-70K-fSNAPc

    Plasmid

    #106987

    Purpose

    open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    U1-70K (SNRNP70 Human)
    

    Use

    Tags

    fSNAP

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1

    Plasmid

    #85070

    Purpose

    Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    PH-PLCD1 (PLCD1 Human)
    

    Use

    Tags

    IRES-sYFP2-PH and mScarlet-H

    Expression

    Mammalian

    Mutation

    PLCD aa 1–170

    Promoter

    CMV

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MAC_C_LTK

    Plasmid

    #187762

    Purpose

    MAC-tagged gene expression of human LTK

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    LTK (LTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPB-TetON-mEGFP-MYOD1_AroPERFECT

    Plasmid

    #215620

    Purpose

    For integration of MYOD1 AroPERFECT T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    MYOD1 AroPERFECT (MYOD1 Human)
    

    Use

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FL-fibulin-3-V5-lentiviral-GFP

    Plasmid

    #29703

    Depositor

    Mariano Viapiano

    Article

    Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

    Insert

    EFEMP1 (EFEMP1 Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

19. AAV-EFS-GFP

    Plasmid

    #170176

    Purpose

    Expresses GFP under the control of EF1a short promoter

    Depositor

    Xiaojun Lian

    Article

    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBiT3.1-N_SCAP:1-905

    Plasmid

    #211228

    Purpose

    Mammalian expression of full-length human SCAP isoform 2. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    SCAP:M1-D905 (SCAP Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    isoform 2. V798I natural variant (VAR_012203)

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge