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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,138 results for: 112

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Showing: 2301 - 2320 of 22138 results

  1. pT7-V5-SBP-C1-HsUpf1_73-1129-siRNAres_P

    Plasmid
    #147192
    Purpose
    Mammalian Expression of HsUpf1_73-1129-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsUpf1_73-1129-siRNAres (UPF1 Human)
    Expression
    Mammalian
    Mutation
    5 non silent mutations A69S, E167G, K282R, A442G…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147192-plasmid-map-sequence-id-289639

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  6. PF3D7_1123500-COMP-blac-flag-his

    Plasmid
    #110987
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    conserved Plasmodium protein (PF3D7_1123500 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110987-plasmid-map-sequence-id-224477

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  8. pTYB-pLAC-L108S/L109S/F112S

    Plasmid
    #48730
    Purpose
    Bacterial expression of Lacritin Protein with Mutation at proteins 108 and 109 Leucine to Serine and 112 from phenylalanine to serine
    Depositor
    Gordon Laurie
    Article
    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6
    Insert
    Lacritin (LACRT Human)
    Tags
    Intein
    Expression
    Bacterial
    Mutation
    Leucine 108 and 109 to Serine; phenylalanine 112 …
    Promoter
    T7
    Available Since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid
    #181791
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181791-plasmid-map-sequence-id-354814

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Showing: 2301 - 2320 of 22138 results