We narrowed to 14,071 results for: cas9

1. Sox1/2/3-2373/+3>nls::Cas9::nls

   Plasmid

   #59989

   Purpose

   Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   nls::Cas9::nls
   

   Use

   CRISPR

   Mutation

   Reverted mutations from dCas9 to wiltdtype

   Promoter

   Ciinte.Sox1/2/3 (SoxB1) -2373/+3

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIVTR(T7A)-Cas9-T2A-mClover3

   Plasmid

   #172293

   Purpose

   In vitro transcription of Cas9-T2A-mClover3 synthetic construct

   Depositor

   Patrik Verstreken

   Insert

   Cas9-T2A-mClover3
   

   Use

   CRISPR

   Promoter

   T7 promoter A-initiating

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

   Plasmid

   #165082

   Purpose

   gRNA 1 of pair B for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. UAS-FRT-GFP-FRT-u(M)Cas9

   Plasmid

   #127388

   Purpose

   Cas9 inducible by removal of a GFP flip-out cassette

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   FRT-GFP-FRT-uORF(M)Cas9
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

   Plasmid

   #159903

   Purpose

   Mutagenesis of Oprk1

   Depositor

   Larry Zweifel

   Insert

   Oprk1 (Oprk1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-SlugCas9-NNG-P2A-puro

   Plasmid

   #214352

   Purpose

   Expresses SlugCas9-NNG and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCas9-NNG and puromycin resistance genes
   

   Expression

   Mammalian

   Mutation

   Q782R/S888R/L906R/N984S/E1012K/K1016I

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRM049 His-TEV-Cas9 triNLS 2C

   Plasmid

   #196245

   Purpose

   Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

   Depositor

   Ross Wilson

   Article

   Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA, His6 (N terminal on backbone), NLS, Nucleopla…

   Expression

   Bacterial

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)

   Plasmid

   #160608

   Purpose

   TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   P35s:dCas9:SRDX:tNOS
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-LTR-dCas9-p65AD-BFP

   Plasmid

   #46913

   Purpose

   Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   dCas9-p65AD-BFP fusion
   Puromycin resistance
   

   Use

   CRISPR and Retroviral

   Tags

   2xNLS, BFP, and VP64 domain

   Expression

   Mammalian

   Promoter

   LTR and PGK

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. NLS-dCas9-HA-NLS-GFP-VP16

    Plasmid

    #183923

    Purpose

    VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    dCas9-GFP-VP16
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMVp-dCas9-3xNLS-VP64 (Construct 1)

    Plasmid

    #55195

    Purpose

    Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VP64

    Expression

    Mammalian

    Promoter

    CMV/hUBC

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pspCas9-Klenow-ST2-com-vector

    Plasmid

    #176235

    Purpose

    Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2T-CMV-evoCjCas9-TadCDd-BlastR

    Plasmid

    #202558

    Purpose

    Mammalian expression of evoCjCas9 TadCDd

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-TadCDd
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCCL-PGK-SPdCas9-BFP-DNMT1

    Plasmid

    #66818

    Purpose

    dCas9 fused to BFP and the human DNMT1 catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dCas9-BFP-DNMT1, catalytic domain (DNMT1 )
    

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid

    #195741

    Purpose

    Base editing for making a pre-stop codon in mmoX using phenol inducible system

    Depositor

    Seung-Goo Lee

    Article

    Jeong et al Chem Eng J, 2023

    Insert

    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Po (phoenol-inducible promoter)

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid

    #217304

    Purpose

    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

    Depositor

    Luke Gilbert

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Promoter

    Ef1alpha

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-UGI-nCas9(H840A)-GSVG

    Plasmid

    #187411

    Purpose

    DddA GSVG variant

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-FLEX-SaCas9-U6-sgTh(2)

    Plasmid

    #209198

    Purpose

    Mutagenesis of Th

    Depositor

    Larry Zweifel

    Article

    Yu et al Neuron. 2021 Apr 21;109(8):1365-1380.e5.

    Insert

    Th (Th Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Promoter

    CMV

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti GFAABC1D-dCas9-KRAB-MeCP2

    Plasmid

    #194701

    Purpose

    Expresses dCas9-KRAB-MeCP2 in Astrocytes

    Depositor

    Timothy Jarome

    Article

    Farrell et al Learn Mem. 2023 Mar 15;30(3):70-73. doi:

    Insert

    GFAABC1D promoter
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    None

    Promoter

    GFAABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV hUbC-dCas9-dMQ1-EGFG

    Plasmid

    #89794

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR and Lentiviral

    Tags

    3*FLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    C141S, TGC-->TCC; S317A, AGC-->GCC

    Promoter

    hUbC

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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