167,985 results

1. pLenti_Ef1a:PE2-P2A-hMLH1dn

   Plasmid

   #184444

   Purpose

   Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

   Depositor

   Bobby Koeleman

   Article

   Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and Ef1A

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rtn3S-GFP

   Plasmid

   #169035

   Purpose

   Express human Rtn3S in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

   Insert

   Rtn3S (RTN3 Human)
   

   Tags

   acGFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHDM-VSV-G

   Plasmid

   #164440

   Purpose

   Encodes VSV-G protein for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   VSV-G
   

   Expression

   Mammalian

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEM047 [T7-assisted CROPseq sgRNA expression vector]

   Plasmid

   #224899

   Purpose

   Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA

   Depositor

   Thomas Norman

   Article

   Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi

   Inserts

   eGFP
   LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-RNF2

   Plasmid

   #126544

   Purpose

   Expresses GFP-tagged RNF2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

   Insert

   RING1B (RNF2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   July 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+MLS-HyPer7

   Plasmid

   #136470

   Purpose

   Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Tags

   Tandem mitochondrial targeting signal of cytochro…

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-hM4D(Gi)-mCherry (AAV2)

   Viral Prep

   #50475-AAV2

   Purpose

   Ready-to-use AAV2 particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   mCherry

   Available Since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pSAM_Ec

   Plasmid

   #102939

   Purpose

   Transposon mutagenesis and Tn-seq

   Depositor

   Matthew Mulvey

   Article

   Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

   Insert

   mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
   

   Expression

   Bacterial

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-CRISPR

   Plasmid

   #160047

   Purpose

   For cloning sgRNA to knockout a specific gene in piggybac plasmid

   Depositor

   Xiaojun Lian

   Article

   Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

   Type

   Empty backbone

   Use

   CRISPR; Piggybac

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. BM00654

    Plasmid

    #155339

    Purpose

    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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