We narrowed to 9,275 results for: Pol;

1. pMpGE_En01

   Plasmid

   #71534

   Purpose

   Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Takayuki Kohchi, Ikuko Hara-Nishimura

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMpGWB405

   Plasmid

   #68670

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   Citrine

   Expression

   Plant

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMpGWB402

   Plasmid

   #68667

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI-MS2V5-PABPC1 MLLEmut

   Plasmid

   #65808

   Purpose

   expression of PABPC1-MS2 fusion. Mutation of C-terminal MLLE domain.

   Depositor

   Niels Gehring

   Article

   Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

   Insert

   PABPC1 (PABPC2 Human)
   

   Tags

   MS2 stem loops and V5

   Expression

   Mammalian

   Mutation

   MLLEmut

   Promoter

   CMV

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMpGWB131

   Plasmid

   #68585

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   Emerald Luc fused with PEST sequence [ELuc(PEST)]

   Expression

   Plant

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMpGWB435

   Plasmid

   #68700

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   TagRFP

   Expression

   Plant

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-CibN-dCas9

   Plasmid

   #60551

   Purpose

   Expresses CibN-dCas9 in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   CibN-dCas9
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMpGWB331

   Plasmid

   #68659

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   Emerald Luc fused with PEST sequence [ELuc(PEST)]

   Expression

   Plant

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMpGWB316

   Plasmid

   #68644

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   Nuclear localization signal-tagged tdTomato (tdTo…

   Expression

   Plant

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pL0_AC-CEN_ARS_HIS

    Plasmid

    #202129

    Purpose

    Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    CEN_ARS_HIS
    

    Use

    Synthetic Biology

    Mutation

    ARS sequence mutated to remove sapI site for dome…

    Available Since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA3.1-dCas9-CibN

    Plasmid

    #60552

    Purpose

    Expresses dCas9-CibN in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    dCas9-CibN
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-SRGAP1FBAR(516a.a.)-EGFP

    Plasmid

    #168505

    Purpose

    Expression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.

    Depositor

    Franck Polleux

    Article

    Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

    Insert

    SRGAP1-FBAR (SRGAP1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-516

    Promoter

    CAG

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. EcPPK1

    Plasmid

    #108850

    Purpose

    Expresses E. coli PPK1 in mammalian cell lines

    Depositor

    Michael Downey

    Article

    Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

    Insert

    Polyphosphate Kinase 1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4/TO h-PKD1 FLS (4107-4303)-GST

    Plasmid

    #83456

    Purpose

    Human PC1-CTp30 expression cassette with a GST-tag (678)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Amino-acids 4107-4303

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pL0_EF-PtBle

    Plasmid

    #202180

    Purpose

    Level 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    ShBle expression cassette
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pPSP6+t

    Plasmid

    #48149

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMpGWB406

    Plasmid

    #68671

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Citrine

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMpGWB431

    Plasmid

    #68696

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Emerald Luc fused with PEST sequence [ELuc(PEST)]

    Expression

    Plant

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNOC-CRISPR

    Plasmid

    #100008

    Purpose

    Expresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genome

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-Nlux

    Promoter

    Ribi promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC-STITCH

    Plasmid

    #129535

    Purpose

    STITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.

    Depositor

    Hideyuki Okano

    Article

    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

    Insert

    STITCH
    

    Use

    CRISPR; Donor template

    Available Since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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