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pCIneo-lambdaN-HA-HsAGO4_C

Plasmid

#146085

Purpose

Mammalian Expression of HsAGO4

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO4 (AGO4 Human)

Expression

Mammalian

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C_C

Plasmid

#146089

Purpose

Mammalian Expression of HsTNRC6C

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C (TNRC6C Human)

Expression

Mammalian

Mutation

3 silent mutations compared to the sequence of Hs…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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HA-RNF4/pRK5

Plasmid

#59743

Purpose

Expresses RNF4 in mammalian cells

Depositor

Xiaolu Yang

Article

Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

Insert

RNF4 (RNF4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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SS-HA-Sun1L-KDEL

Plasmid

#187016

Purpose

Disruption of LINC Complex

Depositor

Jan Lammerding

Article

Lombardi et al J Biol Chem. 2011 Jul 29;286(30):26743-5

Insert

SS-HA-Sun1L-KDEL

Use

Lentiviral

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pGH8 - pRAB-3::mCherry::unc-54utr

Plasmid

#19359

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Prab-3::mCherry

Tags

mCherry

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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mCherry-Nesprin-1αKASH

Plasmid

#187015

Purpose

Disruption of LINC Complex

Depositor

Jan Lammerding

Article

Lombardi et al J Biol Chem. 2011 Jul 29;286(30):26743-5

Insert

Nesprin-1alphaKASH

Use

Lentiviral

Tags

mCherry

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pECFP18aaEYFP

Plasmid

#109330

Purpose

ECFP-EYFP FRET positive control

Depositor

Gabriele Kaminski Schierle

Article

Kaminski et al Methods Mol Biol. 2014;1076:445-54. doi:

Insert

ECFP-EYFP FRET positive control

Expression

Mammalian

Mutation

ECFP linked to EYFP with 18 aa linker GLRSRAQASNS…

Promoter

CMV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pATT-Dest-Cre

Plasmid

#202714

Purpose

Cre recombinase staging plasmid to make transposon insertion libraries.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

Cre

Use

Staging plasmid to make transposon insertion libr…

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Stat2 pBluescript SK (-)

Plasmid

#8705

Depositor

Jim Darnell

Article

Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

Insert

Stat2 (STAT2 Human)

Expression

Bacterial

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pCDF-SeAvs3

Plasmid

#188805

Purpose

Codon optimized SeAvs3 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

SeAvs3

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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YSBE 610 : pHYG_6xHA

Plasmid

#92091

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

6xHA

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCDF-EcAvs4

Plasmid

#188806

Purpose

Codon optimized EcAvs4 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs4

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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YSBE 731 : pHYG_4xFLAG

Plasmid

#92090

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

4xFlag

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLB1080

Plasmid

#37826

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

ATP7B (ATP7B Human)

Tags

mEGFP

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-MBP_F

Plasmid

#146318

Purpose

Mammalian Expression of MBP

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

MBP (MBP Human)

Expression

Mammalian

Mutation

N217S

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLK65

Plasmid

#37257

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

vang-like 2 (VANGL2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr

Plasmid

#19326

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pmyo-3::twk-18(cn110)cDNA

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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pComb3HTT

Plasmid

#63900

Purpose

Backbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.

Depositor

Carlos Barbas

Article

Barbas et al Methods, A companion to Methods in Enzym

Insert

TT Fab

Use

Phage display

Tags

geneIII

Expression

Bacterial

Promoter

lacZ

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pQP-CiRISB

Plasmid

#138978

Purpose

Light-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ68 - Exp5605_Punc-122::GFP::unc-54utr

Plasmid

#19325

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Punc-122::GFP

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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