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pHR-Spy-CD28

Plasmid

#223622

Purpose

Lentiviral expression of human CD28 with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-PDL1-LgBiT

Plasmid

#223619

Purpose

Lentiviral expression of human PD-L1 with LgBiT tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

LgBiT

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28-LgBiT

Plasmid

#223616

Purpose

Lentiviral expression of human CD28 with LgBiT tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

LgBiT

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD86-SNAP

Plasmid

#223613

Purpose

Lentiviral expression of human CD86 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD80-SNAP

Plasmid

#223612

Purpose

Lentiviral expression of human CD80 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28-CLIP

Plasmid

#223610

Purpose

Lentiviral expression of human CD28 with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-CD28

Plasmid

#223600

Purpose

Lentiviral expression of human CD28 with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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LBR(R583Q)-FKBP-GFP

Plasmid

#222423

Purpose

To express a cholesterol synthesis point mutant of LBR (R583Q) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LBR(R583Q) (LBR Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

R583Q

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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LBR(N547D)-FKBP-GFP

Plasmid

#222422

Purpose

To express a cholesterol synthesis point mutant of LBR (N547D) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LBR(N547D) (LBR Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

N547D

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-mCherry-RAB7-S72A

Plasmid

#221556

Purpose

Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

Rab7A (RAB7A Human)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Mutation

Change serine 72 to alanine

Promoter

EF1a

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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