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pCR-Hyg

Plasmid

#158708

Purpose

For cloning and constitutive expression of crRNAs in mycobacterium

Depositor

Yi-Cheng Sun

Article

Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

crRNA

Use

CRISPR and Synthetic Biology; Mycobacteria expres…

Expression

Bacterial

Promoter

Hsp60

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAXY0002

Plasmid

#179835

Purpose

Binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

Depositor

Xiaohan Yang

Article

Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

Type

Empty backbone

Use

Binary vector for plant transformation

Expression

Plant

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pXG237

Plasmid

#141281

Purpose

ATF4 translational reporter

Depositor

Martin Kampmann

Article

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

uORF1/2 of ATF4 followed by mApple

Use

Lentiviral

Tags

mApple

Promoter

CMV

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pDL278_P23-DsRed-Express2

Plasmid

#121505

Purpose

E. coli-streptococcal shuttle vector that expresses rfp

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

DsRed-Express2

Use

E. coli streptococcal shuttle vector

Promoter

P23

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pETM6-mCherry

Plasmid

#66534

Purpose

Expresses mCherry under the control of the Concensus T7 Promoter

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Consensus T7

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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PCMV4-3 HA/IkB-alpha(SS32,36AA)

Plasmid

#24143

Depositor

Warner Greene

Article

Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

Insert

IkB alpha (NFKBIA Human)

Tags

3xHA

Expression

Mammalian

Mutation

S32A S36A

Available Since

March 25, 2010

Availability

Academic Institutions and Nonprofits only

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SpyTag003-sfGFP

Plasmid

#133454

Purpose

Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-sfGFP

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pTOX6

Plasmid

#127451

Purpose

Encodes Tse2 toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

tse2 toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-RelA

Plasmid

#23255

Depositor

Warner Greene

Article

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Insert

p65 (RELA Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

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pSGKP-km

Plasmid

#117233

Purpose

A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Pink Flamindo

Plasmid

#102356

Purpose

biosensor for cAMP in mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Harada et al Sci Rep. 2017 Aug 4;7(1):7351. doi: 10.1

Insert

Pink Flamindo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pKAR2-Br512

Plasmid

#161875

Purpose

Expresses Br512 polymerase in E.coli

Depositor

Andrew Ellington

Article

Maranhao et al medRxiv

Insert

Modified DNA Polymerase I Large Fragment (Br512)

Tags

8x His tag; Villin Headpiece 47 amino acid (HP47)…

Expression

Bacterial

Promoter

T7, tetPA

Available Since

Nov. 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONRPEX18Gm-SceI-pheS

Plasmid

#111609

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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V5-GFP-TurboID-KDEL_pLX208

Plasmid

#202020

Purpose

expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-TurboID-KDEL

Use

Lentiviral

Tags

GFP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCFD5

Plasmid

#73914

Purpose

multiplex gRNA plasmid

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

dU6-3:tRNA:gRNA:tRNA:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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SpyTag003-mKate2

Plasmid

#133452

Purpose

Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mKate2

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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dCas13d-dsRBD

Plasmid

#154938

Purpose

BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

Depositor

Alice Ting

Article

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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NES-mCherry-P4Mx1

Plasmid

#108143

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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LAMP1-APEX2

Plasmid

#215555

Purpose

Stable, inducible expression in lysosome

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

LAMP1-EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-roxSTOProx-ZsGreen

Plasmid

#51274

Purpose

Dre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreen

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

roxSTOProx-ZsGreen

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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