We narrowed to 18,078 results for: Emb
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Plasmid#26537DepositorInsertpre-let-7_PS hairpin
UseRetroviralExpressionMammalianMutation5 nt mutated to make pre-let-7 stem pairing perfe…Available SinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
phRL-TKlk-lin-41_LCS-2U
Plasmid#26543DepositorInsertlin-41_LCS-2U
ExpressionMammalianMutationSeed regions of both target sites are mutated.Available SinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
phRL-TKlk-lin-41_LCS-2G
Plasmid#26544DepositorInsertlin-41_LCS-2G
ExpressionMammalianMutationSeed regions of both target sites are mutated.Available SinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-H2B-mCherry
Plasmid#89766PurposeLentivirus destination vector for stable expression of mCherry-tagged histone H2BDepositorAvailable SinceSept. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
GZnP2 in pcDNA3.1+
Plasmid#161739PurposeCytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)DepositorInsertGZnP2
ExpressionMammalianPromoterCMVAvailable SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
mScarletI3-6DG5-MAPPER
Plasmid#222421PurposeTo express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)DepositorInsertMAPPER
TagsmScarletI3-6DG5ExpressionMammalianAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRPF215
Plasmid#106377PurposeC. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposonDepositorInsertPtet-himar1-Ter(slpA)-transposon
UseE. coli - c. difficile shuttle vectorMutationCodon optimised for C. difficilePromoterPtetAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lyso-B
Plasmid#209873PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddGFP B
TagsLAMP1ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lyso-GA
Plasmid#209874PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddGFP A
TagsLAMP1ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PM-B
Plasmid#209876PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43DepositorInsertddGFP B
TagsTargeting domain of Gap43 MLCCMRRTKQVEKNDDQKIExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_Stargazin-mTurquoise2-iLID
Plasmid#161001PurposeMammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusionDepositorInsertStargazin-mTurquoise2-iLID
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMU-PMr
Plasmid#61180PurposeFluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under AtUBQ10 promoterDepositorInsertAtPIP2a
TagsmCherryExpressionPlantPromoterAtUBQ10Available SinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD-B
Plasmid#209861PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorInsertddGFP B
TagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEx-insertion-tet
Plasmid#172211PurposeFor genetic insertion in tetracyclin-sensitive Prevotella copri strainsDepositorTypeEmpty backboneUseGenetic insertion in prevotella copriAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lyso-RA
Plasmid#209875PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddRFP A
TagsLAMP1ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-CAAX
Plasmid#116856Purposeplasma membrane markerDepositorInsertHRAS
TagsTagBFP2ExpressionMammalianMutationAmino acids 172-189Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGene-TAP-GBP1
Plasmid#138087PurposePlasmid with TAP-GBP1 ORFDepositorInserthuman GBP1 (GBP1 Human)
TagsTandem affinity purification (TAP) tagExpressionMammalianPromoterGal4-E1bAvailable SinceApril 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRE4-Bpa
Plasmid#155342PurposePlasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.DepositorInserthumanized EBpaRS/BstYam
ExpressionMammalianPromoterPGK/U6Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PM-RA
Plasmid#209878PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43DepositorInsertddRFP A
TagsTargeting domain of Gap43 MLCCMRRTKQVEKNDDQKIExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
HRE-doUnaG
Plasmid#201713PurposeHypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequenceDepositorInsertUnaG
ExpressionMammalianAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only