We narrowed to 6,602 results for: mcs

1. pEvol-pAzFRS.2.t1

   Plasmid

   #73546

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAzFRS.2.t1
   pAzFRS.2.t1
   

   Expression

   Bacterial

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. AICSDP-34: TNNI1-mEGFP

   Plasmid

   #114411

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

   Insert

   TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEvol-pAcFRS.2.t1

   Plasmid

   #73544

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAcFRS.2.t1
   pAcFRS.2.t1
   

   Expression

   Bacterial

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Neo_CAG-Cas9

   Plasmid

   #86698

   Purpose

   Cas9 expression vector for targeting to the AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   pCag

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRIP-SFFV-mtagBFP-2A STING V155M

   Plasmid

   #102591

   Purpose

   Lentivector to express STING V155M and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   V155M

   Promoter

   SFFV

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-MYO7A-TAIL

   Plasmid

   #89585

   Purpose

   Expression plasmid for a positive control bait protein in NanoSPD 2.0 assays.

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYO7A (Myo7a Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation encoding amino acids 870-2166 of MYO7A…

   Promoter

   CMV

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-MYO10-HMM-Nanotrap

   Plasmid

   #87255

   Purpose

   Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYO10 (Myo10 Mouse)
   

   Tags

   GFP-nanotrap

   Expression

   Mammalian

   Mutation

   Myo10 is truncated to contain aa.1-941

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFPC1-hVAP-A

   Plasmid

   #104447

   Purpose

   Expression of human VAP-A fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein A (VAPA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Puro_siKO-2TO

   Plasmid

   #86697

   Purpose

   Tetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   H1 2TO

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEvol-pAzFRS.1.t1

    Plasmid

    #73547

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.1.t1
    pAzFRS.1.t1
    

    Expression

    Bacterial

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFPC1-hVAP-B

    Plasmid

    #104448

    Purpose

    Expression of human VAP-B fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein B (VAPB Human)
    

    Tags

    EGFP and HA

    Expression

    Mammalian

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-Rab11-DN (S25N)

    Plasmid

    #101046

    Purpose

    Expresses HA-tagged human Rab11-DN (S25N) in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    RAB11 (RAB11A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S25N

    Promoter

    CMV

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMIR-21 Reporter

    Plasmid

    #71870

    Purpose

    Mammalian expression vector for the analysis of miR-21 activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Reverse complementary sequence of miR-21
    

    Tags

    firefly luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-hygro-STING V155M

    Plasmid

    #102599

    Purpose

    Express STING V155M for IFN-beta Luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    V155M

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-Rab11-WT

    Plasmid

    #101047

    Purpose

    Expresses HA-tagged human Rab11-WT in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    RAB11 (RAB11A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSCV-hygro-STING HAQ

    Plasmid

    #102600

    Purpose

    Express STING HAQ for IFN-beta Luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    R71H-G230A-R293Q (HAQ)

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRIP-SFFV-mtagBFP-2A STING-342STOP

    Plasmid

    #102588

    Purpose

    Lentivector to express STING delta 342 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    delta 342

    Promoter

    SFFV

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-hygro-STING HAQ+V155M

    Plasmid

    #102601

    Purpose

    Express STING V55M HAQ for IFN-beta Luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    V155M R71H-G230A-R293Q

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCIRCgp41-1

    Plasmid

    #74227

    Purpose

    Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    gp41-1 C-intein and gp41-1 N-intein

    Expression

    Bacterial

    Promoter

    T7-lac

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDuEx2-NIR-GECO1

    Plasmid

    #113680

    Purpose

    Bacterial and mammalian expression plasmid for near-infrared fluorescent Ca2+ indicator NIR-GECO1

    Depositor

    Robert Campbell

    Article

    Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

    Insert

    NIR-GECO1
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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