We narrowed to 54,252 results for: plasmid

1. pGEX4T-1-Optimized UBE1L

   Plasmid

   #165099

   Purpose

   ecoli optimized expression of GST-fusion of human Ube1L

   Depositor

   Arthur Haas

   Insert

   UbeE1L (UBA7 Human)
   

   Use

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   codon optimized for E. coli expression

   Promoter

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. ARHGEF2A-c055

   Plasmid

   #205103

   Purpose

   ARHGEF2 protein expression

   Depositor

   Paul Brennan

   Insert

   ARHGEF2 (ARHGEF2 Human)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. ARHGEF2A-c052

   Plasmid

   #205102

   Purpose

   ARHGEF2 protein expression

   Depositor

   Paul Brennan

   Insert

   ARHGEF2 (ARHGEF2 Human)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

   Plasmid

   #51085

   Purpose

   Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

   Depositor

   Jonathan Ting

   Has Service

   AAV Retrograde and AAV1

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   P2A-nls-dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin1

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

   Plasmid

   #51084

   Purpose

   Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

   Depositor

   Jonathan Ting

   Has Service

   AAV Retrograde and AAV1

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   P2A-nls-dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin1

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.2-V5-DEST-hSFRP1-his

   Plasmid

   #189540

   Purpose

   Protein expression of SFRP1 in mammalian cells.

   Depositor

   Haian Fu

   Insert

   secreted frizzled related protein 1 (SFRP1 Human)
   

   Use

   Tags

   his

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

   Plasmid

   #51082

   Purpose

   Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

   Depositor

   Jonathan Ting

   Has Service

   AAV1

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   P2A-nls-dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1a

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-BE4max-P2A-EGFP (pRZ33)

   Plasmid

   #140083

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP

   Depositor

   Keith Joung

   Insert

   Human codon optimized pCAG BE4max with P2A-EGFP
   

   Use

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A (SpCas9)

   Promoter

   CAG

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWPI-IRES-Puro-Ak-ACE2

   Plasmid

   #154985

   Purpose

   Lentiviral vector expressing Human ACE2

   Depositor

   Sonja Best

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221 LRRK2 wt

    Plasmid

    #39529

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Gateway compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. AiP14534 - pAAV-AiE0390m_3xC2-minBglobin-iAChSnFR-Venus-WPRE3-BGHpA (Alias: CN4534)

    Plasmid

    #208164

    Purpose

    Direct-expressing iAChSnFR-Venus in astrocytes

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Insert

    iAChSnFR-Venus
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK GFP Paxillin

    Plasmid

    #50529

    Purpose

    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    Paxillin (PXN Human)
    

    Use

    Tags

    GFP21

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Q-IL6-9

    Plasmid

    #166552

    Purpose

    scFv of a human scaffold targeting Interleukin-6. Antigen coverage aa 28-212 of 212, full-length excl. signal peptide

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, Sortag, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. P48

    Plasmid

    #212155

    Purpose

    Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.

    Depositor

    Kang Zhou

    Insert

    superfolder green fluorescent protein, secondary alcohol dehydrogenase
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR223 CD147, open

    Plasmid

    #149720

    Purpose

    for Gateway cloning of CD147 into destination vector

    Depositor

    Anne-Claude Gingras

    Insert

    CD147 (BSG Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    wt, without stop codon

    Promoter

    Available since

    June 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pGEX6P1 METTL3

    Plasmid

    #200731

    Purpose

    Bacterial expression of GST-Tagged codon optimized human METTL3

    Depositor

    Claudio Alarcón

    Insert

    METTL3 (METTL3 Human)
    

    Use

    Tags

    GST from backbone plasmid

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWPI-IRES-Bla-Ak-ACE2

    Plasmid

    #154981

    Purpose

    Lentiviral vector expressing Human ACE2

    Depositor

    Sonja Best

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. IMBB2.4-pUC57-mini

    Plasmid

    #88845

    Purpose

    plasmid for "Tip Snip" cloning (described in forthcoming Biotechniques article)

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al Biotechniques. 2017 Mar 1;62(3):99-106.

    Type

    Empty backbone

    Use

    Synthetic Biology; Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV CMV FST-FLAG

    Plasmid

    #175167

    Purpose

    Lentiviral expression of human FST-FLAG

    Depositor

    Larry Gerace

    Insert

    FST (FST Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. SOX17-tdTomato-EhB

    Plasmid

    #210467

    Purpose

    Donor plasmid for knock-in tdTomato into the human SOX17 locus

    Depositor

    Kai Wang

    Insert

    SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived hygromycin-NLS-mTagBFP2 selection cassette (SOX17 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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