We narrowed to 3,326 results for: PPL

Showing: 2361 - 2380 of 3326 results

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pEasyG2_hph

Plasmid

#184915

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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OptoProfilin

Plasmid

#208286

Purpose

Expresses Cry2(1-531)-mCherry-Profilin.WT

Depositor

Robert Hughes

Article

Mann et al Chembiochem. 2024 May 2;25(9):e202400007

Insert

Cry2 - mCherry - Profilin.WT (Cry2, PRF1 Mustard Weed, Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Profilin WT (Mus musculus)

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.minBG-EGFP-W3SL_2x-U6-sasgAi14

Plasmid

#231365

Purpose

minBG-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.Ple155-CI-EGFP-W3SL_2x-U6-sasgAi14

Plasmid

#231366

Purpose

Ple155-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ArchT-GFP

Plasmid

#63141

Purpose

AAV-mediated expression of ArchT-GFP under the Syn promoter. Using hGHpA signal.

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin promoter

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(E123A)-EYFP

Plasmid

#35505

Purpose

AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV9

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123A

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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mTFP1-Caveolin-C-10

Plasmid

#55468

Purpose

Localization: Membrane, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

NM_001753 (CAV1 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231364

Purpose

Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-ArchT-GFP

Plasmid

#37807

Purpose

Viral expression of ArchT-GFP for optogenetic inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Promoter

CamKII

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-ArchT-GFP (PV2527)

Plasmid

#99039

Purpose

AAV-mediated expression of ArchT under the CamKII promoter

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

ArchT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CamKII

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-ATRX ADD

Plasmid

#59698

Purpose

Contains histone modification interacting domain (ATRX ADD)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

ATRX ADD (ATRX Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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HTRA1_PDZ_1

Plasmid

#103912

Purpose

Protein expression and purification of HTRA1 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

HTRA1 (HTRA1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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mTFP1-Clathrin-15

Plasmid

#55471

Purpose

Localization: Clathrin Vesicles, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Clathrin (CLTB Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-Alpha-V-Integrin-25

Plasmid

#55465

Purpose

Localization: Focal Adhesions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Alpha-V Integrin (ITGAV Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP1.v1 puro

Plasmid

#87790

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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DLG4_PDZ_3

Plasmid

#103953

Purpose

Protein expression and purification of PSD95 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

DLG4 (DLG4 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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TS-PE2-C-WPRE

Plasmid

#176650

Purpose

Expresses C-terminus of PE2 in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Inserts

C-terminus of PE2
Artificial SA
WPRE
bGH poly(A)

Use

AAV

Tags

SV40 NLS

Expression

Mammalian

Promoter

None

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123A)-mCherry

Plasmid

#35508

Purpose

AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCR2.1-promoter-mIL-6

Plasmid

#109296

Purpose

IL-6 promoter reporter construct

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

murine IL-6 promoter (Il6 Mouse)

Expression

Mammalian

Mutation

CMV promoter in pEGFP-N1 replaced with murine IL-…

Promoter

murine IL-6 promoter

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP1.v2 puro

Plasmid

#87791

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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