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ATeam1.03-nD/nA/pcDNA3

Plasmid

#51958

Purpose

mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Expression

Mammalian

Available Since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-Red

Plasmid

#48249

Purpose

Genetically encoded hydrogen peroxide indicator with red fluorescence.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HYPER-RED

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pBAD-KIRIN1-GR

Plasmid

#107417

Purpose

Bacterial Expression plasmid for potassium ion ratiometric indicator KIRIN1-GR

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1-GR

Expression

Bacterial

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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Ateam1.03-nD/nA/pRSETB

Plasmid

#51960

Purpose

bacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Tags

6xHis

Expression

Bacterial

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-HaloGFP-Na2.4

Plasmid

#228471

Purpose

Mammalian expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

Depositor

Robert Campbell

Article

Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

Insert

HaloGFP-Na2.4

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG2ABC-eIF3Δbgi

Plasmid

#240043

Purpose

Human eIF3k, eIF3l, eIF3e, eIF3f, eIF3h, eIF3d, eIF3m, eIF3a and eIF3c expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3k, l, e, f, h, d, m, a, c

Tags

eIF3a: TEV_3xFLAG, eIF3c: 3C_STREP II, eIF3d: HA

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBbA2C_Ptet::traI

Plasmid

#189575

Purpose

Tra synthase (3OC8-HSL)

Depositor

Daniel Ducat

Article

Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

Insert

Acyl-homoserine-lactone synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Ateam1.03-nD/nA/pENTR1A

Plasmid

#51959

Purpose

Gateway entry vector containing ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Use

Entry vector

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-HASAP

Plasmid

#138325

Purpose

chemigenetic voltage indicator

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Insert

HASAP

Expression

Mammalian

Promoter

CAG

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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Gal:mCherry-uTEV3

Plasmid

#135453

Purpose

Yeast expression of uTEV3 fused to mCherry

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

mCherry-uTEv3

Tags

mCherry

Expression

Yeast

Mutation

S219V mutation improves stability , I138T/S153N/T…

Promoter

Gal

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-KIRIN1

Plasmid

#107414

Purpose

Bacterial Expression plasmid for potassium ion ratiometric indicator KIRIN1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1

Expression

Bacterial

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-HArcLight

Plasmid

#138326

Purpose

chemigenetic voltage indicator

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Insert

HArcLight

Expression

Mammalian

Promoter

CAG

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-KIRIN1-GR

Plasmid

#107418

Purpose

Mammalian cell expression plasmid for potassium ion ratiometric indicator KIRIN1-GR

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1-GR

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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U6-AsCas12f-sgRNA-v2

Plasmid

#204639

Purpose

Expression of trancated AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

trancated AsCas12f sgRNA (sgRNA-v2)

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pUC-TALO8

Plasmid

#83409

Purpose

TRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeast

Depositor

Toshi Tsukiyama

Article

Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

Insert

TRP-ARS-8xLacO

Expression

Yeast

Mutation

Please see depositor comments below

Promoter

TRP1

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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piGECInano-N1

Plasmid

#186190

Purpose

Expression of small FRET-based near-infrared genetically-encoded calcium indicator in mammalian cells

Depositor

Vladislav Verkhusha

Article

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-GCaMP6fu

Plasmid

#86235

Purpose

Express fluorescent calcium indicator GCaMP6fu in mammalian cells

Depositor

Katalin Torok

Article

Helassa et al Sci Rep. 2016 Dec 6;6:38276. doi: 10.103

Insert

GCaMP6fu

Expression

Mammalian

Mutation

W43Y D395A

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Orange_CaMBI_110

Plasmid

#124094

Purpose

orange calcium-modulated bioluminescent indicator, 110nM

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Orange CaMBI 110

Expression

Mammalian

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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CMV:eGFP-CaM-uTEV1Δ(220-242)

Plasmid

#135461

Purpose

Mammalian expression of uFLARE protease component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

eGFP-CaM-uTEV1Δ

Tags

eGFP, V5

Expression

Mammalian

Mutation

S219V mutation improves stability and S153N impro…

Promoter

CMV

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-Green Falcan0.3

Plasmid

#199704

Purpose

green fluorescent indicator for cAMP imaging

Depositor

Tetsuya Kitaguchi

Article

Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

Insert

Green Falcan0.3

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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