173,361 results
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Plasmid#207499PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertFAS/OX40, NY-ESO-1_TCR (FAS Human)
UseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest-1.7kbfabp6-eGFP-pA-CrymCherry
Plasmid#159088PurposeLR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA insertsDepositorInsertsUseFluorescent reporterPromoter1.7kb fabp6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pANAP-P2A-eRF(E55D)
Plasmid#241290PurposeAllows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses tRNACUA-Leu, AnapRS, and dominant negative ribosomal release factorDepositorInsertthe amber suppressor tRNACUA-Leu
ExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMVAvailable SinceDec. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-TadCBEd-ivt-template
Plasmid#193843PurposeTemplate for in vitro transcription of TadCBEd (with SpCas9)DepositorInsertTadCBEd with SpCas9
UseIn vitro transcription templateAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiSSTe10_ChR2_mCherry (AAV PHP.eB)
Viral Prep#213815-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiSSTe10_ChR2_mCherry (#213815). In addition to the viral particles, you will also receive purified pAAV_BiSSTe10_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the SST interneuron-targeting enhancer E10. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorTagsmCherryAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIG2-Flag-mNuak1 WT
Plasmid#168507PurposeFor expression of Nuak1 (AMPK-related kinase also called ARK5/Omphk1)DepositorAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast POLE1 N-ISCmut
Plasmid#160803PurposeExpress N-terminal ISC mutant POLE1DepositorInsertPOLE1 N-ISCmut (POLE Human)
UseRetroviralTags3xFLAGMutationshPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…Available SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_EGFP
Plasmid#25899PurposeGateway entry vector containing EGFP.DepositorInsertEGFP
UseGateway entry vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4-5HT3 HC IRES EGFP
Plasmid#119740PurposeChemogenetic activator expression plasmidDepositorExpressionMammalianMutationL131G, Q139L, Y217F, R420Q, R424D, R428APromoterCAGAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptSKII+ U6-sgRNA(F+E) empty
Plasmid#74707PurposeEncodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequenceDepositorInsertU6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
ExpressionMammalianAvailable SinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only