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  5. pSKI

    Plasmid
    #232484
    Purpose
    pSKI can be used for single-copy transgene expression within the Universal SKI System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.
    Depositor
    Carlos Silva García
    Article
    Dinneen et al bioRxiv [Preprint]. 2024 Dec 10:2024.12.
    Insert
    pSKI
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232484-plasmid-map-sequence-id-464988

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  14. pAGD236

    Plasmid
    #219497
    Purpose
    pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter
    Depositor
    Seth Shipman
    Article
    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.
    Insert
    sfGFP, Eco1 msr, Eco1 RT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219497-plasmid-map-sequence-id-435233

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  15. pAGD248

    Plasmid
    #219498
    Purpose
    Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)
    Depositor
    Seth Shipman
    Article
    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.
    Insert
    Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219498-plasmid-map-sequence-id-435231

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  17. pJYDNgp

    Plasmid
    #162453
    Purpose
    Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-2Glinker-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA nad myc and eUnaG2 fluorescent protein
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162453-plasmid-map-sequence-id-321460

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Showing: 331 - 360 of 3620 results