We narrowed to 10,998 results for: ENA;

1. pKJ235

   Plasmid

   #217493

   Purpose

   Mammlian expression of MmFnuc for genome editing

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

   Insert

   MmFnuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKJ318

   Plasmid

   #217495

   Purpose

   Mammlian expression of MmFnuc guide RNA

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

   Insert

   MmFnuc guide RNA scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pfMDH_K381A

   Plasmid

   #67732

   Purpose

   Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed lysine 381 to alanine

   Promoter

   T7

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pfMDH_N300D

   Plasmid

   #67728

   Purpose

   Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed asparagine 300 to aspartate

   Promoter

   T7

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pfMDH_R373A

   Plasmid

   #67731

   Purpose

   Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed arginine 373 to alanine

   Promoter

   T7

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pfMDH_N300S

   Plasmid

   #67729

   Purpose

   Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed asparagine 300 to serine

   Promoter

   T7

   Available Since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pETM6-T7-VHH-GFPminimizer

   Plasmid

   #202488

   Purpose

   Expresses the nanobody GFP minimizer with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody GFP minimizer
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSMART-HCKan-yibDp-VHH-VCAM1

   Plasmid

   #202471

   Purpose

   Expresses the nanobody VCAM1 after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody VCAM1
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGS-21a-PcAad1

   Plasmid

   #163039

   Purpose

   expression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium

   Depositor

   Jean-Marie Francois

   Article

   Yang et al BMC Microbiol. 2012 Jun 28;12:126. doi:

   Insert

   aryl-alcohol dehydrogenase
   

   Use

   Tags

   GST and His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HUW-TetO-hNanog

    Plasmid

    #25700

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

    Insert

    Nanog (NANOG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pCOLA-Gent-EM7-Erv1p

    Plasmid

    #202484

    Purpose

    Expresses sulfhydryl oxidase (Erv1p) constitutively

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    EM7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM6-Amp-T7-roGFP

    Plasmid

    #202464

    Purpose

    Expresses redox sensitive GFP (roGFP) with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    reduction-oxidation sensitive green fluorescent protein
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcF-1

    Plasmid

    #131023

    Purpose

    Single spacer array with spacer #1 for FnCas12a.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Insert

    single spacer array for FnCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRL1124

    Plasmid

    #70690

    Purpose

    provides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Inserts

    avaiM
    eco47iiM
    ecot22iM
    

    Use

    Bacterial shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJZ001

    Plasmid

    #120230

    Purpose

    Contains intB13 integrase casette for knock-in to pCAP-BAC backbone

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    intB13 integrase casette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    bla-g717a (amp resistance gene)

    Promoter

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSMART-HCKan-yibDp-VHH72

    Plasmid

    #202467

    Purpose

    Expresses the nanobody VHH72 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody VHH72
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSMART-HCKan-yibDp-VHH-H6antivenin

    Plasmid

    #202470

    Purpose

    Expresses the nanobody H6 antivenin after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody H6 antivenin
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSMART-HCKan-yibDp-VHH-1B5

    Plasmid

    #202472

    Purpose

    Expresses the nanobody 1B5 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody 1B5
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSMART-HCKan-yibDp-VHH-2E7

    Plasmid

    #202473

    Purpose

    Expresses the nanobody 2E7 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody 2E7
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSMART-HCKan-yibDp-VHH-3E3

    Plasmid

    #202474

    Purpose

    Expresses the nanobody 3E3 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody 3E3
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge