Addgene

We narrowed to 502 results for: Fap;

Showing: 221 - 240 of 502 results

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pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine

Plasmid

#50471

Purpose

Gi-coupled hM4D-IRES-mCitrine under the control of GFAP promoter

Depositor

Bryan Roth

Insert

hM4D(Gi)-IRES-mCitrine (CHRM4 Human)

Use

AAV

Tags

HA

Expression

Mutation

See supplemental documents for DREADD mutations

Promoter

GFAP

Available since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-GFAP(long)-FLEX-GCaMP7f

Plasmid

#189612

Purpose

Cre-dependent GCaMP7f under the GFAP promoter

Depositor

Martin Riccomagno

Article

Agnew-Svoboda et al Cell Rep Methods. 2022 Aug 22;2(8):10027

Insert

GCaMP7f

Use

AAV and Cre/Lox

Tags

GCaMP7f

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-hChR2(H134R)-mCherry

Plasmid

#27055

Purpose

AAV expression of humanized ChR2(H134R) fused to mCherry, for optogenetic excitation

Depositor

Karl Deisseroth

Insert

hChR2(H134R)

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

H134R

Promoter

GFAP

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-hFAPP1 PH domain

Plasmid

#58723

Purpose

Expresses GFP-Tagged human FAPP1 PH domain in yeast

Depositor

Tim Levine

Article

Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

Insert

Four-phosphate-adaptor protein 1 PH domain (PLEKHA3 Human)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pGP-AAV-GFAP-iGABASnFR2n-WPRE

Plasmid

#218872

Purpose

AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2n

Use

AAV

Tags

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

GFAP

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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hGFAP-NLS-HA-Dre-P2A-BFP

Plasmid

#51277

Purpose

Co-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS-HA-Dre-P2A-BFP

Use

Tags

Expression

Mutation

Promoter

hGFAP

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pTYF-sGFAP-LOx-IRES-tdTomato

Plasmid

#166919

Purpose

Express the bacterial lactate oxidase (LOx) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Insert

Lactate oxidase

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

sGFAP

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-MFAP2

Plasmid

#185555

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting MFAP2

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

MFAP2 gRNAs

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-jGCaMP7c variant 1513

Plasmid

#173159

Purpose

Expresses jGCaMP7c variant 1513 in astrocytes

Depositor

Vincent Vialou, Hervé Chneiweiss

Insert

jGCaMP7c variant 1513-WPRE

Use

AAV

Tags

Expression

Mutation

Promoter

GFAP

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pXCX-sGFAP-LMO-IRES-tdTomato

Plasmid

#178312

Purpose

Express the bacterial lactate 2-monooxygenase (LMO) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

Lactate 2-monooxygenase

Use

Adenoviral

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

sGFAP

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP(ABC1D)-ECS-SpyCatcher003

Plasmid

#234545

Purpose

to express SpyCatcher on the extracellular side of astrocytic plasmamembrane

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-SC

Use

AAV

Tags

Expression

Mutation

None

Promoter

mGFAP(ABC1D)

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP(ABC1D)-ECS-dSpyCatcher003

Plasmid

#234546

Purpose

to express dSpyCatcher on the extracellular side of astrocytic plasmamembrane

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-dSC

Use

AAV

Tags

Expression

Mutation

None

Promoter

mGFAP(ABC1D)

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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GFAP-MCS-WPRE-SV40 - GCH31

Plasmid

#230829

Purpose

AAV cloning vector for expression under the GFAP promoter.

Depositor

Kimberly Ritola

Type

Empty backbone

Use

AAV; Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-GFAP [N206A/8R-rat IgG2a]

Plasmid

#225421

Purpose

Mammalian Expression Plasmid of anti-GFAP (Human) rat IgG2a R-mAb. Derived from hybridoma N206A/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GFAP (Homo sapiens) recombinant (Mouse) monoclonal antibody. (GFAP Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-804_HA-GD2-28z_CAR_BATF-TFAP4

Plasmid

#207491

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF-TFAP4, HA-GD2-28z_CAR (BATF Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-806_HA-GD2-28z_CAR_RFP-TFAP4

Plasmid

#207493

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-851_HA-GD2-28z_HA-TFAP4

Plasmid

#207501

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

HA-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pXCX-sGFAP-DLDH-IRES-tdTomato

Plasmid

#178311

Purpose

Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

D-lactate dehydrogenase

Use

Adenoviral

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

sGFAP

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1alpha-HA-TFAP4(E135A/S139A)

Plasmid

#110159

Purpose

Lentiviral vector for expression of human TFAP4 (E135A/S139A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

Insert

TFAP4 (transcription factor AP-4) (TFAP4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

E135A/S139A

Promoter

CMV

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hGFAP-Pink Flamindo-WPRE-SV40

Plasmid

#178790

Purpose

AAV vector to express the red cAMP indicator in astrocytes

Depositor

Tetsuya Kitaguchi

Article

Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

Insert

Pink Flamindo

Use

AAV

Tags

Expression

Mutation

Promoter

hGFAP

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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