Addgene

We narrowed to 876 results for: GGCT;

Showing: 221 - 240 of 876 results

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pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPR-dCas9

Plasmid

#125687

Purpose

Gene knockdown for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spdCas9, sgRNA casette

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

ermE*/tipA

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgPenk

Plasmid

#159916

Purpose

Mutagenesis of Penk

Depositor

Larry Zweifel

Insert

Penk (Penk Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCR8-attL3-pegRNA-attL2

Plasmid

#213052

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attL1-pegRNA-attR3

Plasmid

#213051

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA3.1

Plasmid

#64118

Purpose

Vector for expression of St1 sgRNA3.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA3.1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCR8-attL5-pegRNA-attR3

Plasmid

#213054

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attR4-pegRNA-attR3

Plasmid

#213056

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attL5-pegRNA-attL4

Plasmid

#213055

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attL1-pegRNA-attR5

Plasmid

#213053

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - CTRL sgRNA

Plasmid

#70662

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a non-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS597

Plasmid

#188884

Purpose

dpy-10 sgRNA expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

PU6(K09B11.12)::dpy-10 sgRNA (dpy-10 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pDropVn

Plasmid

#184873

Purpose

Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pX330-NEAT1pr_v1

Plasmid

#97081

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1pr_v1

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_T2

Plasmid

#184864

Purpose

pSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_T1

Plasmid

#184863

Purpose

pSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO mouse shRNA 2 raptor

Plasmid

#21340

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 13. ():.

Insert

raptor shRNA 2 (Rptor Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
pegRNA_LMNA-1

Plasmid

#199273

Purpose

pegRNA used to induce LMNA (c.1824C > T) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LMNA 1824CtoT pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C6

Plasmid

#160457

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7-Promoter_C6

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AksgRNA

Plasmid

#121955

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b single chimeric gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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