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pRDAX-VALENTIA-010 LifeAct-Neptune//BsaI

Plasmid

#130191

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

LifeAct-Neptune

Use

Synthetic Biology

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-027 LifeAct-mVenus//BsaI

Plasmid

#130208

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

LifeAct-mVenus

Use

Synthetic Biology

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-009 LifeAct-mVenus//BsaI

Plasmid

#130190

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

LifeAct-mVenus

Use

Synthetic Biology

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-008 LifeAct-Eos//BsaI

Plasmid

#130189

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

LifeAct-Eos

Use

Synthetic Biology

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-001 LifeAct-Sirius//BsaI

Plasmid

#130182

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

LifeAct-Sirius

Use

Synthetic Biology

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

Plasmid

#175526

Purpose

Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag11 and Tomm20-HaloTag7

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

Plasmid

#175261

Purpose

labelling of microtubule plus end and F-actin structures

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)

Tags

mCherry (on iLifeact) and mNeonGreen (on EB3)

Expression

Mammalian

Mutation

None (wt)

Promoter

pTub-alpha1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

Plasmid

#229826

Purpose

Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet-I

Use

AAV

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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S. rosetta Col- cDNA amplified library

Pooled Library

#62843

Purpose

amplified library of Salpingoeca rosetta

Depositor

Nicole King

Article

Fairclough et al Genome Biol. 2013 Feb 18;14(2):R15. doi:

Expression

Bacterial

Species

Salpingoeca rosetta

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only
S. rosetta Col+ cDNA amplified library

Pooled Library

#62844

Purpose

amplified library of Salpingoeca rosetta from a colony rich culture which included the colony inducing bacteria Algoriphagus machipongonensis

Depositor

Nicole King

Article

Fairclough et al Genome Biol. 2013 Feb 18;14(2):R15. doi:

Expression

Bacterial

Species

Salpingoeca rosetta

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only
GIL-6

Plasmid

#213389

Purpose

Expresses a chimeric cytokine between IL-6 and LIF

Depositor

Jurgen Scheller

Article

Rafii et al J Biol Chem. 2024 May;300(5):107251. doi

Insert

GIL-6

Tags

IgG1-Fc-tag and Myc-tg

Expression

Mammalian

Promoter

T7

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GIL-11 myc TEV TwinStrep

Plasmid

#199627

Purpose

Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

Depositor

Jurgen Scheller

Article

Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

Insert

GIL-11

Tags

TwinStrep-tag

Expression

Mammalian

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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MultiSite Gateway plasmid toolKit

Plasmid Kit

#1000000050

Purpose

Cloning kit for constructing fully-customizable three-part fusion proteins, based on MultiSite Gateway from Life Technologies. 12 promoter-less Gateway entry clones, N- and C-term flanking modules

Depositor

Tomas Santalucia

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
BJ5183 cells

Bacterial Strain

#16398

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Bacterial Resistance

Streptomycin

Species

Escherichia coli

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
AdEasier-1 cells (strain)

Bacterial Strain

#16399

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Bacterial Resistance

Ampicillin and Streptomycin

Species

Escherichia coli

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
bSLS.114

Bacterial Strain

#191530

Purpose

Contains a knockout gene in the E. coli strain BL21-AI

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Bacterial Resistance

None

Species

Escherichia coli

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only
bMS.346

Bacterial Strain

#220588

Purpose

Increase editing efficiency of retron and oligo recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Bacterial Resistance

None

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only
scAAV-TTR-mFgf15

Plasmid

#190594

Purpose

AAV construct containing Fgf15 cds under control of TTR promoter

Depositor

Roel Nusse

Article

Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

Insert

Fgf15 (Fgf15 Mouse)

Use

AAV

Expression

Mammalian

Promoter

Ttr

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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N1-G-Ca-FLITS

Plasmid

#191465

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

G-Ca-FLITS

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDYH5

Plasmid

#52032

Purpose

Expression of GFP-tagged DAF-16 in C. elegans

Depositor

Meng-Qiu Dong

Article

Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

Insert

DAF-16 (daf-16 Nematode)

Tags

GFP and HIS

Expression

Worm

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only