We narrowed to 1,166 results for: Ncl

1. pJMP1263

   Plasmid

   #119264

   Purpose

   B. subtilis attTn7 site and flanking sequence cloned into pACYC

   Depositor

   Jason Peters, Oren Rosenberg, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   B. subtilis attTn7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJMP1106

   Plasmid

   #119249

   Purpose

   construction intermediate

   Depositor

   Jason Peters, Oren Rosenberg, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLEX-FLAG-RHEB-IRES-Puro

   Plasmid

   #120576

   Purpose

   Expresses Flag-RHEB fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   RHEB (RHEB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP_EHMT1V09

   Plasmid

   #228813

   Purpose

   Expression of mouse EHMT1 with deletion of aa 1-31

   Depositor

   Shravanti Rampalli

   Article

   Biligiri et al PLOS Biology 22(11): e3002871.

   Insert

   EHMT1 (Ehmt1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deletion of aa 1-31

   Promoter

   Available since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNDGG010

   Plasmid

   #231315

   Purpose

   Vector part for BEVA; BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNDGG009

   Plasmid

   #231314

   Purpose

   Vector part for BEVA; BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FLAG NC

   Plasmid

   #214675

   Purpose

   Negative control plasmid for splicing reporters consisting of MCP fused FLAG epitope tags

   Depositor

   Eugene Yeo

   Article

   Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   FLAG
   

   Use

   Tags

   MCP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOME0135

   Plasmid

   #178397

   Purpose

   KSHV Kaposincluster-RevCompl-Syn

   Depositor

   Dirk Dittmer

   Article

   Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

   Insert

   Kaposincluster-RevCompl-SYN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOME234M

   Plasmid

   #178416

   Purpose

   Reverse compl sequence of KSHV Kaposincluster-RevCompl-Syn for pcDNA3.1-

   Depositor

   Dirk Dittmer

   Article

   Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

   Insert

   Reverse complement Kaposincluster-RevCompl-SYN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOME0235M

    Plasmid

    #178417

    Purpose

    KSHV Reverse compl Sequence Kaposincluster_RevCompl-KapATGdel_Syn

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

    Insert

    ReverseComplement-Kaposincluster_RevCompl_KapATGdel_Syn
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCR8_TaNAM-A1

    Plasmid

    #124574

    Purpose

    TaNAM-A1 CDS in the Gateway-compatible pCR8 vector.

    Depositor

    Cristobal Uauy

    Article

    Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

    Insert

    TaNAM-A1
    

    Use

    Topo gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX-FHH-MAPKAP1RBD

    Plasmid

    #120710

    Purpose

    Expresses Flag-HA-6xHIS-MAPKAP1 RBD fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MAPKAP1 RBD
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Amino acids 279-353

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRK5-HA-Ubiquitin-KO

    Plasmid

    #17603

    Purpose

    Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    KO: all lysines mutated to arginines

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDECKO_TFRC_C (with mCherry)

    Plasmid

    #72627

    Purpose

    Expresses two gRNAs targeting the TFRC promoter

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward TFRC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p6352 MSCV-CMV-CMV-Flag-HA-JMJD6

    Plasmid

    #31358

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    JMJD6 (JMJD6 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p6351 MSCV-CMV-CMV-Flag-HA-NSD3

    Plasmid

    #31357

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    NSD3 (NSD3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3-HA-Akt1

    Plasmid

    #73408

    Purpose

    Expresses human AKT1 with a N-terminus HA tag

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-OSBP-PH

    Plasmid

    #49571

    Purpose

    expresses the plekstrin homology domain of OSBP in mammalian cells

    Depositor

    Marci Scidmore

    Article

    Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

    Insert

    OSBP-PH (AA 91-179) (OSBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJMP1339

    Plasmid

    #119271

    Purpose

    for cloning new sgRNAs

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJMP1337

    Plasmid

    #119270

    Purpose

    for cloning new sgRNAs

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge