We narrowed to 9,087 results for: Pol;

1. pCAG-BiPOLES-mRuby3

   Plasmid

   #227780

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons fused to mRuby3

   Depositor

   Hillel Adesnik

   Insert

   BiPOLES-mRuby3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGGF-bGH polyA-G

   Plasmid

   #218902

   Purpose

   golden gate cloning

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   bGH polyA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-AN-HA_WT POLI

   Plasmid

   #201440

   Purpose

   Expresses human DNA polymerase iota with an N-terminal HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Coding sequence has been optimised for expression…

   Promoter

   CMV

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJM919_WT His POLI

   Plasmid

   #201444

   Purpose

   Low copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Coding sequence has been optimised for expression…

   Promoter

   lacIq

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcBAC3-POLY-pnRFP

   Plasmid

   #201680

   Purpose

   Boost the expression of pnRFP in mammalian cells (e.g., macrophage RAW 264.7 cells)

   Depositor

   Huiwang Ai

   Article

   Pang et al ACS Chem Biol. 2023 Jun 16;18(6):1388-13

   Insert

   813
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. RS314-U6(PolII-Lsm)

   Plasmid

   #200772

   Purpose

   Pol II expression of U6 Lsm

   Depositor

   Aaron Hoskins

   Article

   Lipinski et al RNA. 2022 Dec;28(12):1606-1620. doi: 10.

   Insert

   Gal-SNR6-II
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCK005 ubi:mCerulean_ubb-polyA

   Plasmid

   #195979

   Purpose

   Expression construct

   Depositor

   Alexa Burger, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   ubi:mCerulean
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRE3GV-somBiPOLES-mKate2

   Plasmid

   #192579

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of tTA-positive neurons

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GV

   Available since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRE3GV-somBiPOLES-mCerulean

   Plasmid

   #192578

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of tetTag-positive neurons

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GV

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CaMKII-somBiPOLES-eGFP

    Plasmid

    #192583

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa(0.4)

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. px459-Pole4 KO sgRNA

    Plasmid

    #186935

    Purpose

    Pole4 KO in mouse ES cells

    Depositor

    Zhiguo Zhang

    Article

    Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

    Insert

    Pole4 KO sgRNA (Pole4 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. px459-Pole3 KO sgRNA

    Plasmid

    #186934

    Purpose

    Pole3 KO in mouse ES cells

    Depositor

    Zhiguo Zhang

    Article

    Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

    Insert

    Pole3 KO sgRNA (Pole3 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-sgPole#2/Cre

    Plasmid

    #173610

    Purpose

    Expresses a Pole-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Pole (Pole Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-sgPole#1/Cre

    Plasmid

    #173609

    Purpose

    Expresses a Pole-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Pole (Pole Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-POLBHR-eGFP

    Plasmid

    #176064

    Purpose

    Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    EGFP-PolB (POLB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    pUC with endogenous SapI mutated out, PAM mutatio…

    Promoter

    N/A

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiGuide-PolB1-puro

    Plasmid

    #177146

    Purpose

    Lentiviral vector expressing PolB-gRNA-1 and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolBgRNA1 (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV-U6+27Linear-2, PolyU(0)

    Plasmid

    #166994

    Purpose

    Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination module:PolyU(0)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV-U6+27-Linear-1, PolyU(0)

    Plasmid

    #166992

    Purpose

    Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination module:PolyU(0)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. GLY, Linear-3, PolyU(1)

    Plasmid

    #166988

    Purpose

    Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLNYtRNA promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination module:PolyU(1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GLY, Linear-3, PolyU(4)

    Plasmid

    #166989

    Purpose

    Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination module:PolyU(4)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge