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Plasmid#195314PurposeGenetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, double-flowed in AAV vector under the promoter hSynDepositorInsertSpikeyGi2
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsin promoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.ArcLightD.WPRE.SV40
Plasmid#100037PurposeAAV expression of ArcLightD driven by hSynap promoterDepositorInsertArcLightD
UseAAVTagsExpressionMammalianMutationPromoterhSynapAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_VP1_RaPID_r1c3
Plasmid#65723PurposeProduce random mCherry insertion in AAV2 VP3 region of VP1; ITRs in VP1 onlyDepositorInsertmCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA
Plasmid#205281PurposeCaMP used to measuring calcium changes in neurons, while nls-mRuby is employed to specifically identify cortical GABAergic neurons. This dual labeling approach allows for the simultaneous observation of neuronal activity using GCaMP and the identification of a specific subset of neurons using nls-mRuby, focusing specifically on cortical GABAergic neurons.DepositorInsertGCaMP6s
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.ZAC.GFAP.iAChSnFR
Plasmid#137962PurposeExpresses iAChSnFR under GFAP promoterDepositorArticleInsertiAChSnFR
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_AAVS1
Plasmid#141212PurposepDECKO_mCherry_AAVS1_186. Targeting, non-essential locus controlDepositorInsertgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.iGABASnFR.R205A
Plasmid#112162PurposeGABA SensorDepositorInsertiGABASnFR.R205A
UseAAVTagsExpressionMutationR205A (no GABA binding)PromoterhSynapsinAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_12xTEAD_luc2
Plasmid#173004Purposefirefly luciferase reporter vector with 12x clustered TEAD binding sitesDepositorInsert12xTEAD-luc2
UseAAV and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSynapsin1_NOPLight1
Plasmid#195580PurposeExpresses NOPLight1 in neuronsDepositorInsertNOPLight1
UseAAVTagsExpressionMammalianMutationPromoterhuman Synapsin-1Available sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.iGABASnFR.F102Y.Y137L
Plasmid#112161PurposeGABA SensorDepositorInsertiGABASnFR.F102Y.Y137L
UseAAVTagsExpressionMutationF102Y.Y137LPromoterhSynapsinAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.iGABASnFR.F102Y.Y137L
Plasmid#112174PurposeGABA SensorDepositorInsertiGABASnFR.F102Y.Y137L
UseAAVTagsExpressionMutationF102Y.Y137LPromoterGFAPAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.iGluSnFr.WPRE.SV40
Plasmid#98932PurposeCre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
psAAVS1-T2
Plasmid#60543PurposeGFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activitiesDepositorInsertProtoSpacer AAVS1-T2 (AAVS1 Human)
UseCRISPR; KanTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2
Plasmid#171938PurposeExpresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteinsDepositorInsertApex2, mRuby2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV2_hSyn_Phobos_CA_Citrine
Plasmid#98218PurposeSlow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct Phobos_C128A gene
UseAAVTagsCitrineExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…Promoterhuman synapsinAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Cbh_DIO_hTyr
Plasmid#205287PurposeInduces NM-like pigment accumulation in catecholaminergic neuronsDepositorInserthTyrosinase
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40
Plasmid#100038PurposeAAV expression of FLEXed ArcLightD driven by hSynap promoterDepositorInsertArcLightDco
UseAAVTagsExpressionMammalianMutationPromoterhSynapAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40
Plasmid#98931PurposeCre dependent AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_GFP_AAVS1
Plasmid#141211PurposepDECKO_GFP_AAVS1_1801. Targeting, non-essential locus controlDepositorInsertgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mClover3_AAVS1_sgRNA_2
Plasmid#155099Purposelentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only