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pGEL636

Plasmid

#189830

Purpose

The T-DNA backbone of Cas12j2 for transcriptional activation in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Promoter

pZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET47b-T7-dAsCas12f

Plasmid

#204641

Purpose

Expression of an inactive AsCas12f in E. coli

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

inactive AsCas12f

Tags

6xHis

Expression

Bacterial

Mutation

D225A

Promoter

T7

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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3185 pBS Bax cDNA

Plasmid

#8754

Depositor

Stanley Korsmeyer

Article

Knudson et al Science 1995 Oct 6;270(5233):96-9.

Insert

Bax (Bax Mouse)

Expression

Bacterial

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pET21a.MJ0285

Plasmid

#11304

Depositor

Sung-Hou Kim

Article

Kim et al J Struct Biol. 1998 Jan . 121(1):76-80.

Insert

small heat shock protein (MJ_0285 M. jannaschii)

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pIRES.Puro.EGFP

Plasmid

#24176

Depositor

Robert Sobol

Article

Jelezcova et al Mutat Res. 2010 Apr 1;686(1-2):57-67. do

Insert

EGFP

Expression

Mammalian

Mutation

x

Available Since

April 9, 2010

Availability

Academic Institutions and Nonprofits only
pBAD mPlum

Plasmid

#91773

Purpose

Bacterial expression of a fluorescent protein, monomeric Plum (mPlum)

Depositor

Erik Rodriguez, Roger Tsien

Article

Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;10

Insert

monomeric Plum

Tags

no tag

Expression

Bacterial

Promoter

araBAD

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pPP378

Plasmid

#158797

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-1 (D371A)

Expression

Bacterial

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pPP384

Plasmid

#158799

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-3 (D413A)

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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3177 pSFFV-neo HA Bax alpha

Plasmid

#8810

Depositor

Stanley Korsmeyer

Article

Knudson et al Science 1995 Oct 6;270(5233):96-9.

Insert

Bax alpha (Bax Mouse)

Tags

HA

Expression

Mammalian

Available Since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-C67Y

Plasmid

#10982

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-C67Y (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 200 G to A in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-C70Y

Plasmid

#10983

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-C70Y (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 209 G to A in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-H80Y

Plasmid

#10984

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-H80Y (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 238 C to T in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-D260N

Plasmid

#10985

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-D260N (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 778 G to A in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-D261N

Plasmid

#10986

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-D261N (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 781 G to A in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-R320C

Plasmid

#10987

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-R320C (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 958 C to T in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-E1230K

Plasmid

#10990

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-E1230K (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 3688 G to A in the RPB1 ORF resulting in…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pCMV2 HuOpt Bxb1

Plasmid

#242869

Purpose

Mammalian expression of Bxb1 Integrase

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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SM281_EF1A-dEnAsCas12a-P300-T2A-EGFP

Plasmid

#244216

Purpose

Mammalian expression of dEnAsCas12a-P300 with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PROM5_MprbcL_short_chlo

Plasmid

#165161

Purpose

L0 part - promoter

Depositor

Jim Haseloff

Article

Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

Insert

Marchantia polymorpha rbcL promoter region

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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5UTR_Mprbcl_chlo

Plasmid

#163934

Purpose

L0 part - 5'UTR

Depositor

Jim Haseloff

Article

Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

Insert

Marchantia polymorpha rbcL 5'UTR

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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