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Plasmid#37192DepositorInsertEDD C2768A (UBR5 Human)
UseTags6x His Tag and FLAGExpressionMammalianMutationChanged Cysteine 2768 to Alanine; K503R (please s…PromoterSV40Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-F1888L
Plasmid#69010Purposeactivating MTOR mutationDepositorInsertMTOR-F1888L (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Phenylalanine 1888 to LeucinePromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pD21-hgAPOAI
Plasmid#124962PurposeExpress ApoAI in the liverDepositorInserthuman APOAI gene (LPA Human)
UseAdenoviralTagsExpressionMutationPromoterhuman APOAIAvailable sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
scADGFP
Plasmid#114434PurposeEpilepsy promoter enhanced with Arc elements driving GFP with SV40E polyADepositorInsertGreen fluorescent protein
UseAAVTagsER export sequence FCYENEVExpressionMammalianMutationPromoterNovel synthetic activity-dependent promoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,Δ42a] mut
Plasmid#26577DepositorInsertCACNA1D (Cacna1d Rat)
UseTagsExpressionMammalianMutationContains exons 8a, 11, and 31b.Exons 32 and 42a a…PromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-D2512H
Plasmid#69017Purposeactivating MTOR mutationDepositorInsertMTOR-D2512H (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Aspartate 2512 to HistidinePromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-RoninDHSA-FLAG-IRES-Neo
Plasmid#28021DepositorInsertRonin (THAP11 Human)
UseTagsFLAGExpressionMammalianMutationDHSA mutation (Y246A) of the previously defined H…PromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-PGC-1alpha2
Plasmid#45502DepositorInsertPGC-1alpha Isoform2 (Ppargc1a Mouse)
UseTags6xHis and FlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-RFP-UCR1C
Plasmid#67582PurposeExpresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cellsDepositorInsertC-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)
UseTagsFLAG and TagRFPExpressionMammalianMutationAmino acid residues 80-136 of PDE4D3PromoterAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pShuttle CMV RGECO-TnT
Plasmid#124643PurposeMyofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination.DepositorInsertRGECO-TnT (TNNT2 Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP
Plasmid#154787PurposeExpresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domainDepositorInsertAdenosine deaminse (Adar Fly, Synthetic)
UseLentiviralTagsGFP, V5, and stdMCPExpressionMammalianMutationSynonymized serine to prevent autoediting of ADAR…PromoterUBCAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
MOM603
Plasmid#133242Purposeexpresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cellsDepositorInsertkif1a (KIF1A Human)
UseTagsStrepII tag and mScarletExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,Δ42a]
Plasmid#49332PurposeRepaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
UseTagsExpressionMammalianMutationContains exons 8a, 11, and 31b.Exons 32 and 42a a…PromoterCMVAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-3xFLAG-YAP1-S127A
Plasmid#42239DepositorInsertYAP1 (YAP1 Human)
UseGatewayTags3xFLAGExpressionMutationSerine 127 changed to alaninePromoternoneAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
lenti-HA-mBec1
Plasmid#99505Purpose3rd generation lentiviral expression of wild-type Becn1DepositorInsertBeclin 1 (Becn1 Mouse)
UseLentiviralTagsHAExpressionMutationPromoterCMVAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
UCP3 promoter EP1
Plasmid#71743PurposeContains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2; used to quantify promoter activityDepositorInsertUncoupling Protein 3 promoter (Ucp3 Mouse)
UseTagsLuciferaseExpressionBacterialMutationContains 7kb of the murine UCP3 gene encompassing…PromoterUCP3Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-HsAIDSc
Plasmid#60810Purposeto express human AID recoded for yeast codon usageDepositorInsertAID (AICDA Human)
UseTagsMycExpressionYeastMutationcodon optimized for yeastPromoterGAL1Available sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pShuttle CMV RGECO-TnI
Plasmid#124642PurposeMyofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombinationDepositorInsertRGECO-TnI (TNNI3 Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRC/RSV-rat Agt
Plasmid#122104PurposeExpression of angiotensinogenDepositorInsertangiotensinogen (Agt Rat)
UseTagsExpressionMammalianMutationPromoterRSV promoterAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
MYH11-NanoLuc-2A-tdTomato
Plasmid#132569PurposeDonor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 alleleDepositorInsertHuman MYH11 knockin homology arms (MYH11 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only