167,948 results

1. pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

   Plasmid

   #67986

   Purpose

   Cas9 activity reporter with mCherry and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Inserts

   U6gRNA cassette, PGKmCherryABFP cassette, WPRE
   Guide RNA targeting modified BFP
   

   Use

   CRISPR and Lentiviral

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-TRE3g-mNeon-WPRE-pA

   Plasmid

   #192064

   Purpose

   mNeonGreen expression with TRE3g promoter

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   mNeonGreen
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TRE3g

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-nlsABACUS2-400n

   Plasmid

   #203726

   Purpose

   Gateway entry vector for the the nlsABACUS2-400n biosensor for (+)-Abscisic acid

   Depositor

   Alexander Jones

   Article

   Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

   Insert

   nlsABACUS2-400n
   

   Use

   Synthetic Biology

   Tags

   Myc

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (AAV1)

   Viral Prep

   #100853-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (#100853). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven, Cre-dependent GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Promoter

   Syn

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

5. Ub-G76V-GFP

   Plasmid

   #11941

   Depositor

   Nico Dantuma

   Article

   Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

   Insert

   Ub-G76V-GFP
   

   Expression

   Mammalian

   Mutation

   Ubiquitin fused to N-terminus of GFP. Glycine 76 …

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST17-UnaG

   Plasmid

   #203485

   Purpose

   For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

   Insert

   UnaG
   

   Expression

   Bacterial and Plant

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-H2B-iRFP720

   Plasmid

   #128961

   Purpose

   Lentiviral plasmid for expression of far red iRFP720 nuclear marker

   Depositor

   Carlos Carmona-Fontaine

   Insert

   Human histone H2B (H2BC21 Human)
   

   Use

   Lentiviral

   Tags

   iRFP720

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACYC-Taq

   Plasmid

   #102793

   Purpose

   Expresses Taq DNA polymerase under WT T7 promoter

   Depositor

   Andrew Ellington

   Article

   Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

   Insert

   Taq DNA polymerase
   

   Expression

   Bacterial

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-GFP (AAV9-X1.1)

   Viral Prep

   #37825-AAV9-X1.1

   Purpose

   Ready-to-use AAV9-X1.1 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV were produced with the AAV9-X1.1 serotype, which permits efficient transduction of brain endothelial cells. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   GFP

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

10. pJL056

    Plasmid

    #177168

    Purpose

    Expresses wildtype MRE11 in human cells

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Insert

    MRE11 (MRE11 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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