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plIG-110_CD19-BBz_CAR_TF_Library

Pooled Library

#207477

Purpose

CD19-BBz CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-132_CD19-28z_CAR_TF_and_SR_Library

Pooled Library

#207481

Purpose

CD19-28z CAR in combination with 129 surface receptors, 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knoc

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
pCAGGS-3HA-Dennd4c

Plasmid

#246276

Purpose

Rab10 GEF

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

3HA-Dennd4c (Dennd4c Mouse)

Tags

3HA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-TRP1

Plasmid

#229228

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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L0-I11 PhiC31-NLS GG1-GG4

Plasmid

#195894

Purpose

Level 0 PhiC31 inegrase NLS with GG1 GG4 Golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 integrase

Use

Synthetic Biology

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I12 PhiC31-NLS GG2-GG4

Plasmid

#195895

Purpose

Level 0 PhiC31 inegrase NLS with GG2 GG4 Golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 integrase

Use

Synthetic Biology

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST tol2 UbB polyA

Plasmid

#188701

Purpose

Gateway destination vector containing the zebrafish ubiquitin B polyadenylation sequence

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1-FERM

Plasmid

#187371

Purpose

Expresses human Plekhh1-FERM labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1-FERM

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1-CTer

Plasmid

#187369

Purpose

Expresses human Plekhh1-CTer labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1-CTer

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1-NTer

Plasmid

#187368

Purpose

Expresses human Plekhh1-NTer labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1-NTer

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pSP64T-ythdf3-3xflag

Plasmid

#164490

Purpose

Expresses zebrafish ythdf3 with 3x flag tag, for mRNA injection

Depositor

Antonio Giraldez

Article

Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

Insert

ythdf3 (ythdf3 Zebrafish)

Use

Production of mrna for zebrafish injection.

Tags

3x-flagtag

Promoter

SP6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR207-OEP7(1–50)

Plasmid

#100601

Purpose

Gateway entry vector for AtOEP7(1–50)

Depositor

Yutaka Kodama

Article

Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

Insert

OEP7(1–50) (OEP7 Mustard Weed)

Use

Gateway Cloning

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pPHAGE-EF1α-Luc-C20orf24-3’UTR-VAR

Plasmid

#128507

Purpose

Lentiviral constitutive expression of Firefly luciferase under control of 3'UTR of human C20orf24 with variant from COX deficiency patient.

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pB-puro-mU6-RtcAshRNA1

Plasmid

#126613

Purpose

Expresses shRNA against human RtcA under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

RtcA shRNA-1

Use

RNAi

Promoter

mouse U6 promoter

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pB-puro-mU6-RtcAshRNA2

Plasmid

#126614

Purpose

Expresses shRNA against human RtcA under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

RtcA shRNA-2

Use

RNAi

Promoter

Mouse U6 promoter

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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C113S KVS-1-HA

Plasmid

#113070

Purpose

Expresses worm KVS-1 C113S in mammalian cells

Depositor

Federico Sesti

Article

Cai et al Nat Neurosci. 2009 May;12(5):611-7. doi:

Insert

C113S K+ voltage sensitive channel subunit 1 (kvs-1 Nematode)

Tags

HA

Expression

Mammalian

Mutation

C113S

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114(G145A)

Plasmid

#111319

Purpose

snu114(G145A)

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Expression

Yeast

Mutation

snu114 G145A

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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Mmadhc

Plasmid

#74662

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

Mmadhc (MMADHC Human)

Tags

His-6-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
ZP47 Nlk1

Plasmid

#16895

Depositor

Randall Moon

Article

Thorpe et al Development. 2004 Jun;131(12):2899-909.

Insert

Nlk1 (nlk1 Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pCAGGS-3HA-TBC1D30

Plasmid

#246269

Purpose

Rab8 GAP

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

3HA-TBC1D30 (Tbc1d30 Rat)

Tags

3HA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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