We narrowed to 2,498 results for: ERK

1. p2CT-His-MBP-Cam_Cas13a_WT

   Plasmid

   #91869

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   CamCas13a (CamC2c2) WT
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2CT-His-MBP-Ere_Cas13a_WT

   Plasmid

   #91868

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   EreCas13a (EreC2c2) WT
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Sc NybbR eRF1 (WT)

   Plasmid

   #175414

   Purpose

   Encodes His-MBP-TEV-ybbR-WT eRF1

   Depositor

   Joseph Puglisi

   Article

   Lawson et al Science. 2021 Aug 20;373(6557):876-882.

   Insert

   eRF1 (SUP45 Budding Yeast)
   

   Use

   Tags

   His-MBP-TEV and ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. EcNusGNTD

   Plasmid

   #113123

   Purpose

   Expresses N-terminus of E. coli NusG

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   NusG
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Deleted residues 120-181

   Promoter

   T7

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHL-sec

   Plasmid

   #99845

   Purpose

   Mammalian expression with secretion signal sequence

   Depositor

   Edith Yvonne Jones

   Article

   Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   ephrin b2 (EFNB2 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQE30-His-PheRS

   Plasmid

   #124116

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21a-GlyRS-His

   Plasmid

   #124110

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMJ915

   Plasmid

   #69090

   Purpose

   Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

   Depositor

   Jennifer Doudna

   Article

   Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

   Insert

   cas9
   

   Use

   Tags

   MBP, hexa His, and two SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p2CT-His-MBP-Lwa_Cas13a_WT

   Plasmid

   #91865

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LwaCas13a
   

   Use

   CRISPR

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EcNusGFL

    Plasmid

    #113122

    Purpose

    Expresses full-length E. coli NusG

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    NusG (nusG Escherichia coli)
    

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJCC_039 FnCas12a

    Plasmid

    #171666

    Purpose

    For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    FnCas12a
    

    Use

    Tags

    6X His and sortase A sorting motif

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-Wnt4

    Plasmid

    #35910

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt4 (WNT4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent Mutation C (492) --> T

    Promoter

    CMV

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSNAPf-Nup43

    Plasmid

    #98276

    Purpose

    mammalian expression of SNAPf-Nup43

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    Nup43 (NUP43 Human)
    

    Use

    Tags

    SNAPf-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNup133-Ypet

    Plasmid

    #98283

    Purpose

    mammalian expression of Nup133-Ypet

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    Nup133 (NUP133 Human)
    

    Use

    Tags

    YPet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Sc eRF3∆165N (WT)

    Plasmid

    #175418

    Purpose

    Encodes His-MBP-TEV-WT eRF3∆165N

    Depositor

    Joseph Puglisi

    Article

    Lawson et al Science. 2021 Aug 20;373(6557):876-882.

    Insert

    eRF3 (SUP35 Budding Yeast)
    

    Use

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Mutation

    ∆165N

    Promoter

    T7

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAC-dCLK

    Plasmid

    #33153

    Depositor

    Michael Rosbash

    Article

    McDonald et al Mol Cell Biol. 2001 Feb;21(4):1207-17.

    Insert

    CLK (Clk Fly)
    

    Use

    Retroviral

    Tags

    6xHIS and V5

    Expression

    Insect

    Mutation

    Promoter

    Actin 5C

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pKEW336-MBP-TEV-MbCas12a-33362

    Plasmid

    #115670

    Purpose

    Expresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purification

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    MbCas12a
    

    Use

    CRISPR

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNAP

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p2CT-His-MBP-Lbu_C2c2_R1079A_K1080A

    Plasmid

    #91861

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) R0179A/K1080A
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    K1080A

    Promoter

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p2CT-His-MBP-Lbu_C2c2_E299A

    Plasmid

    #91855

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) E299A
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    E299A

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-Wnt4-V5

    Plasmid

    #35929

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt4 (WNT4 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Silent Mutation C (492) --> T

    Promoter

    CMV

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge