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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,523 results for: golden gate

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Showing: 2381 - 2400 of 5523 results

  3. pBFC1171

    Plasmid
    #231994
    Purpose
    CRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Publication
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Insert
    crRNA of dRfxCas13d
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pJEx (Jungle Express)
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    231994-plasmid-map-sequence-id-468506

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  6. pHIE096 CMV Bxb1

    Plasmid
    #139252
    Purpose
    Encodes a Bxb1 recombinase for Landing Pad integration.
    Depositor
    Joshua Leonard
    Insert
    Bxb1 Recombinase
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    139252-plasmid-map-sequence-id-325167

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  8. pOPS0382

    Plasmid
    #133232
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Publication
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available since
    April 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    133232-plasmid-map-sequence-id-287534

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  9. pMH220

    Plasmid
    #122836
    Purpose
    dCas9 with GreenGate B-C flanking sequences
    Depositor
    Markus Schmid
    Publication
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    Use
    Golden gate compatible cloning vector
    Mutation
    Contains D10A and H840A mutations
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122836-plasmid-map-sequence-id-239365

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  10. pGGY003

    Plasmid
    #48867
    Purpose
    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.
    Depositor
    Jan Lohmann
    Publication
    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:
    Type
    Empty backbone
    Use
    Golden gate compatible plant transformation vector
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    48867-plasmid-map-sequence-id-71302

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  17. pSLQ14901

    Plasmid
    #239276
    Purpose
    Expresses gG1 for pertubing endogenous human GAPDH mRNA via CRISPR-TO
    Depositor
    Stanley Qi
    Publication
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PspCas13b-hGAPDH-gRNA1 (GAPDH Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239276-plasmid-map-sequence-id-474385

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  18. SpRY-Tad-CBE(ACC3)

    Plasmid
    #235082
    Purpose
    Expresses SpRY-Tad-CBE(ACC3) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(ACC3)-SpRY Cas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235082-plasmid-map-sequence-id-466790

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Showing: 2381 - 2400 of 5523 results